Deletion of the NR4A nuclear receptor NOR1 in hematopoietic stem cells reduces inflammation but not abdominal aortic aneurysm formation.
BMC Cardiovasc Disord
; 17(1): 271, 2017 Oct 18.
Article
em En
| MEDLINE
| ID: mdl-29047330
ABSTRACT
BACKGROUND:
The NR4A3 orphan nuclear hormone receptor, NOR1, functions as a constitutively active transcription factor to regulate inflammation, proliferation, and cell survival during pathological vascular remodeling. Inflammatory processes represent key mechanisms leading to abdominal aortic aneurysm (AAA) formation. However, a role of NOR1 in AAA formation has not been investigated previously.METHODS:
Inflammatory gene expression was analyzed in bone marrow-derived macrophages isolated from NOR1-deficient mice. Low-density lipoprotein receptor-deficient (LDLr-/-) mice were irradiated and reconstituted with hematopoietic stem cells obtained from NOR1-/- or wild-type littermate mice. Animals were infused with angiotensin II and fed a diet enriched in saturated fat to induce AAA formation. Quantification of AAA formation was performed by ultrasound and ex vivo measurements.RESULTS:
Among 184 inflammatory genes that were analyzed, 36 genes were differentially regulated in LPS-treated NOR1-deficient macrophages. Albeit this difference in gene regulation, NOR1-deficiency in hematopoietic stem cells did not affect development of AAA formation in bone marrow-derived stem cell transplanted LDLr-deficient mice.CONCLUSION:
NOR1 deletion induced differential inflammatory gene transcription in macrophages but did not influence AAA formation in mice.Palavras-chave
Texto completo:
1
Bases de dados:
MEDLINE
Assunto principal:
Receptores dos Hormônios Tireóideos
/
Células-Tronco Hematopoéticas
/
Receptores de Esteroides
/
Deleção de Genes
/
Aneurisma da Aorta Abdominal
/
Proteínas de Ligação a DNA
/
Proteínas do Tecido Nervoso
Limite:
Animals
Idioma:
En
Revista:
BMC Cardiovasc Disord
Assunto da revista:
ANGIOLOGIA
/
CARDIOLOGIA
Ano de publicação:
2017
Tipo de documento:
Article
País de afiliação:
Estados Unidos