A Super-Resolution Probe To Monitor HNO Levels in the Endoplasmic Reticulum of Cells.
Anal Chem
; 89(22): 12087-12093, 2017 11 21.
Article
em En
| MEDLINE
| ID: mdl-29069900
ABSTRACT
Selective detection of nitroxyl (HNO), which has recently been identified as a reactive nitrogen species, is a challenging task. We report a BODIPY-based luminescence ON reagent for detection of HNO in aqueous solution and in live RAW 264.7 cells, based on the soft nucleophilicity of the phosphine oxide functionality toward HNO. The probe shows high selectivity to HNO over other reactive oxygen/nitrogen and sulfur species. Luminescence properties of the BODIPY-based chemodosimetric reagent make it an ideal candidate for use as a reagent for super-resolution structured illumination microscopy. The viability of the reagent for biological in vivo imaging application was also confirmed using Artemia as a model.
Texto completo:
1
Bases de dados:
MEDLINE
Assunto principal:
Retículo Endoplasmático
/
Corantes Fluorescentes
/
Óxidos de Nitrogênio
Limite:
Animals
Idioma:
En
Revista:
Anal Chem
Ano de publicação:
2017
Tipo de documento:
Article
País de afiliação:
Índia