Nonsteroidal Anti-Inflammatory Drug-Induced Leaky Gut Modeled Using Polarized Monolayers of Primary Human Intestinal Epithelial Cells.
ACS Infect Dis
; 4(1): 46-52, 2018 01 12.
Article
em En
| MEDLINE
| ID: mdl-29094594
ABSTRACT
The intestinal epithelium provides a critical barrier that separates the gut microbiota from host tissues. Nonsteroidal anti-inflammatory drugs (NSAIDs) are efficacious analgesics and antipyretics and are among the most frequently used drugs worldwide. In addition to gastric damage, NSAIDs are toxic to the intestinal epithelium, causing erosions, perforations, and longitudinal ulcers in the gut. Here, we use a unique in vitro human primary small intestinal cell monolayer system to pinpoint the intestinal consequences of NSAID treatment. We found that physiologically relevant doses of the NSAID diclofenac (DCF) are cytotoxic because they uncouple mitochondrial oxidative phosphorylation and generate reactive oxygen species. We also find that DCF induces intestinal barrier permeability, facilitating the translocation of compounds from the luminal to the basolateral side of the intestinal epithelium. The results we outline here establish the utility of this novel platform, representative of the human small intestinal epithelium, to understand NSAID toxicity, which can be applied to study multiple aspects of gut barrier function including defense against infectious pathogens and host-microbiota interactions.
Palavras-chave
Texto completo:
1
Bases de dados:
MEDLINE
Assunto principal:
Permeabilidade da Membrana Celular
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Anti-Inflamatórios não Esteroides
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Células Epiteliais
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Mucosa Intestinal
Limite:
Humans
Idioma:
En
Revista:
ACS Infect Dis
Ano de publicação:
2018
Tipo de documento:
Article
País de afiliação:
Estados Unidos