Bone Formation Using Cross-Linked Chitosan Scaffolds in Rat Calvarial Defects.
Implant Dent
; 27(1): 15-21, 2018 Feb.
Article
em En
| MEDLINE
| ID: mdl-29189415
ABSTRACT
PURPOSE:
To investigate the osteoconductive effect of a chitosan scaffold in a rat skull defect model. Previous publications have demonstrated the osteoinductive properties as scaffold materials with growth factors; however, whether chitosan alone has osteoconductive ability is unclear. This study used cross-linked chitosan scaffolds for in vivo evaluation of scaffold-supported bone regeneration in rat calvarial defects using histopathological analysis and examination of alkaline phosphatase (ALP), calcium, phosphorus, and calcitonin serum levels. MATERIALS ANDMETHODS:
Scaffolds were made of cross-linked chitosan. After the defect was filled with the scaffold, the periosteum was carefully repositioned and sutured to stabilize the scaffold. The effects of the scaffold on wound repair were examined microscopically. Morphological radiographic and histopathological analyses of wound repair ratios were performed at 3 and 4 weeks after the defects were made.RESULTS:
Using the cross-linked chitosan biomaterial of the wounds. The amount of regenerated bone measured was significantly greater in the chitosan-treated group than in the control group. The ALP level in the chitosan group at 4 weeks was higher than at baseline and at the 4-week follow-up in the control group (P < 0.05).CONCLUSIONS:
The results show that cross-linked chitosan has an osteoconductive effect on bone regeneration in vivo.
Texto completo:
1
Bases de dados:
MEDLINE
Assunto principal:
Osteogênese
/
Crânio
/
Quitosana
/
Alicerces Teciduais
Tipo de estudo:
Diagnostic_studies
/
Prognostic_studies
Limite:
Animals
Idioma:
En
Revista:
Implant Dent
Assunto da revista:
ODONTOLOGIA
Ano de publicação:
2018
Tipo de documento:
Article
País de afiliação:
Taiwan