Deletion of from Streptococcus pyogenes. Results in Hypervirulence in a Mouse Model of Sepsis and is LuxS Independent.
Pol J Microbiol
; 66(1): 17-24, 2017 Mar 30.
Article
em En
| MEDLINE
| ID: mdl-29359701
ABSTRACT
Group A Streptococcus (GAS) is a Gram-positive human pathogen that causes a variety of diseases ranging from pharyngitis to life-threatening streptococcal toxic shock syndrome. Recently, several global gene expression analyses have yielded extensive new information regarding the regulation of genes encoding known and putative virulence factors in GAS. A microarray analysis found that transcription of the GAS gene M5005_Spy_1343 was significantly increased in response to interaction with human polymorphonuclear leukocytes. M5005_Spy_1343 is predicted to encode a member of the LysR family of transcriptional regulators and is located upstream of a putative operon containing six genes. Five of these genes have sequence similarity to genes involved in short-chain fatty acid metabolism, whereas the sixth gene (luxS) is found in many bacterial species and is involved in quorum sensing. Unexpectedly, inactivation of the M5005_Spy_1343 gene resulted in hypervirulence in an intraperitoneal mouse model of infection. Increased virulence was not due to changes in luxS gene expression. We postulate that short-chain fatty acid metabolism is involved in GAS pathogenesis.
Palavras-chave
Texto completo:
1
Bases de dados:
MEDLINE
Assunto principal:
Infecções Estreptocócicas
/
Streptococcus pyogenes
/
Liases de Carbono-Enxofre
/
Proteínas de Bactérias
/
Deleção de Genes
/
Sepse
Tipo de estudo:
Prognostic_studies
Limite:
Animals
Idioma:
En
Revista:
Pol J Microbiol
Assunto da revista:
MICROBIOLOGIA
Ano de publicação:
2017
Tipo de documento:
Article
País de afiliação:
Polônia