MiR-137-3p rescue motoneuron death by targeting calpain-2.
Nitric Oxide
; 74: 74-85, 2018 04 01.
Article
em En
| MEDLINE
| ID: mdl-29413901
ABSTRACT
Brachial plexus root avulsion (BPRA) is a type of injury that leads to motor function loss as a result of motoneurons (MNs) degeneration. Here we identified that the reduced expression of rat miR-137-3p in the ventral horn of spinal cord was associated with MNs death. However, the pathophysiological role of miR-137-3p in root avulsion remains poorly understood. We demonstrated that the calcium-activated neutral protease-2 (calpain-2) was a direct target gene of miR-137-3p with miR-137-3p binding to the 3'-untranslated region of calpain-2. Silencing of calpain-2 suppressed the expression of neuronal nitric oxide synthase (nNOS), a primary source of nitric oxide (NO). After avulsion 2 weeks, up-regulation of miR-137-3p in the spinal cord reduced calpain-2 levels and nNOS expression inside spinal MNs, resulting in an amelioration of the MNs death. These events provide new insight into the mechanism by which upregulation of miR-137-3p can impair MN survival in the BPRA.
Palavras-chave
Texto completo:
1
Bases de dados:
MEDLINE
Assunto principal:
Calpaína
/
MicroRNAs
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Neurônios Motores
Limite:
Animals
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Humans
Idioma:
En
Revista:
Nitric Oxide
Assunto da revista:
BIOQUIMICA
/
QUIMICA
Ano de publicação:
2018
Tipo de documento:
Article