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Fluorescence-linked immunosorbent assay for detection of phenanthrene and its homolog.
Sun, Yu; Li, Yansong; Meng, Xingyu; Qiao, Bin; Hu, Pan; Meng, Xianmei; Lu, Shiying; Ren, Honglin; Liu, Zengshan; Zhou, Yu.
Afiliação
  • Sun Y; Key Laboratory of Zoonoses Research, Ministry of Education, Institute of Zoonosis, College of Veterinary Medicine, Jilin University, Changchun 130062, PR China; Grain and Oil Food Processing Key Laboratory of Jilin Province, Jilin Business and Technology College, Changchun 130507, PR China.
  • Li Y; Key Laboratory of Zoonoses Research, Ministry of Education, Institute of Zoonosis, College of Veterinary Medicine, Jilin University, Changchun 130062, PR China.
  • Meng X; Key Laboratory of Zoonoses Research, Ministry of Education, Institute of Zoonosis, College of Veterinary Medicine, Jilin University, Changchun 130062, PR China; State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 15
  • Qiao B; Key Laboratory of Zoonoses Research, Ministry of Education, Institute of Zoonosis, College of Veterinary Medicine, Jilin University, Changchun 130062, PR China.
  • Hu P; Key Laboratory of Zoonoses Research, Ministry of Education, Institute of Zoonosis, College of Veterinary Medicine, Jilin University, Changchun 130062, PR China.
  • Meng X; Grain and Oil Food Processing Key Laboratory of Jilin Province, Jilin Business and Technology College, Changchun 130507, PR China.
  • Lu S; Key Laboratory of Zoonoses Research, Ministry of Education, Institute of Zoonosis, College of Veterinary Medicine, Jilin University, Changchun 130062, PR China.
  • Ren H; Key Laboratory of Zoonoses Research, Ministry of Education, Institute of Zoonosis, College of Veterinary Medicine, Jilin University, Changchun 130062, PR China.
  • Liu Z; Key Laboratory of Zoonoses Research, Ministry of Education, Institute of Zoonosis, College of Veterinary Medicine, Jilin University, Changchun 130062, PR China.
  • Zhou Y; Key Laboratory of Zoonoses Research, Ministry of Education, Institute of Zoonosis, College of Veterinary Medicine, Jilin University, Changchun 130062, PR China; Beijing Hongwei Science & Technology Co., Ltd, Beijing 100000, PR China; Jiangsu Co-innovation Center for Prevention and Control of Imp
Anal Biochem ; 547: 45-51, 2018 04 15.
Article em En | MEDLINE | ID: mdl-29458034
ABSTRACT
A competitive fluorescence-linked immunosorbent assay (FLISA) was developed using rhodamine B isothiocyanate (RBITC) as the model fluorescent dye conjugate monoclonal antibody (McAb) for detection of Phe and its homolog (acenaphthene, fluorene, fluoranthene, pyrene and indeno [1,2,3-cd] pyrene) in water samples. The detection range of the assay for Phe was from 2.10 to 91.95 ng/mL. The limit of detection was 1.05 ng/mL, which was approximately 2-fold lower than that of traditional ic-ELISA. Compared with traditional ic-ELISA, more than 70 min was saved because of only one immunoreaction step was needed to accomplish the assay. The average recoveries of Phe and its homolog from domestic water, contaminated water and natural water were 100.7%, 100.8% and 101.2% respectively. The accuracy and precision of the developed FLISA were validated with GC-MS/MS. There were good correlation between the two methods from tap water, contaminated water and river water samples were 0.9994, 0.9935 and 0.9967, respectively. The results suggested that the proposed FLISA could be a potential alternative format for rapid, sensitive, and quantitative detection of Phe and its homolog in environmental water.
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Texto completo: 1 Bases de dados: MEDLINE Assunto principal: Fenantrenos / Poluentes da Água / Água / Imunofluorescência / Anticorpos Monoclonais Murinos Tipo de estudo: Diagnostic_studies Idioma: En Revista: Anal Biochem Ano de publicação: 2018 Tipo de documento: Article

Texto completo: 1 Bases de dados: MEDLINE Assunto principal: Fenantrenos / Poluentes da Água / Água / Imunofluorescência / Anticorpos Monoclonais Murinos Tipo de estudo: Diagnostic_studies Idioma: En Revista: Anal Biochem Ano de publicação: 2018 Tipo de documento: Article