Recommendations for mRNA analysis of micro-dissected glomerular tufts from paraffin-embedded human kidney biopsy samples.

Bockmeyer, Clemens L; Wittig, Juliane; Säuberlich, Karen; Selhausen, Philipp; Eßer, Marc; Zeuschner, Philip; Modde, Friedrich; Amann, Kerstin; Daniel, Christoph

Bockmeyer, Clemens L; Wittig, Juliane; Säuberlich, Karen; Selhausen, Philipp; Eßer, Marc; Zeuschner, Philip; Modde, Friedrich; Amann, Kerstin; Daniel, Christoph.

Afiliação

Bockmeyer CL; Department of Nephropathology, Institute of Pathology, Friedrich-Alexander University Erlangen-Nuremberg, Krankenhausstraße 8-10, 91054, Erlangen, Germany. bockmeyer@gmail.com.
Wittig J; Institute of Pathology, Hannover Medical School, Hannover, Germany. bockmeyer@gmail.com.
Säuberlich K; Institute of Pathology, University Hospital of Cologne, Cologne, Germany.
Selhausen P; Institute of Pathology, University Hospital of Cologne, Cologne, Germany.
Eßer M; Department of Nephropathology, Institute of Pathology, Friedrich-Alexander University Erlangen-Nuremberg, Krankenhausstraße 8-10, 91054, Erlangen, Germany.
Zeuschner P; Institute of Pathology, University Hospital of Cologne, Cologne, Germany.
Modde F; Institute of Pathology, University Hospital of Cologne, Cologne, Germany.
Amann K; Institute of Pathology, Hannover Medical School, Hannover, Germany.
Daniel C; Department of Nephropathology, Institute of Pathology, Friedrich-Alexander University Erlangen-Nuremberg, Krankenhausstraße 8-10, 91054, Erlangen, Germany.

BMC Mol Biol ; 19(1): 2, 2018 03 13.

Article em En | MEDLINE | ID: mdl-29534701

Assuntos

Perfilação da Expressão Gênica/normas; Rim/patologia; Microdissecção e Captura a Laser/métodos; RNA Mensageiro/análise; Adulto; Biópsia; Feminino; Humanos; Rim/química; Masculino; Pessoa de Meia-Idade; Especificidade de Órgãos; Inclusão em Parafina; Adulto Jovem

Palavras-chave

Endogenous controls; FFPE; Housekeeping gene; Kidney biopsies; LCM; Laser capture micro-dissection; Laser microdissection; Reference transcript

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Texto completo: 1 Bases de dados: MEDLINE Assunto principal: RNA Mensageiro / Perfilação da Expressão Gênica / Microdissecção e Captura a Laser / Rim Limite: Adult / Female / Humans / Male / Middle aged Idioma: En Revista: BMC Mol Biol Assunto da revista: BIOLOGIA MOLECULAR Ano de publicação: 2018 Tipo de documento: Article País de afiliação: Alemanha

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