Protein Storage Vacuoles Originate from Remodeled Preexisting Vacuoles in Arabidopsis thaliana.
Plant Physiol
; 177(1): 241-254, 2018 05.
Article
em En
| MEDLINE
| ID: mdl-29555788
ABSTRACT
Protein storage vacuoles (PSV) are the main repository of protein in dicotyledonous seeds, but little is known about the origins of these transient organelles. PSV are hypothesized to either arise de novo or originate from the preexisting embryonic vacuole (EV) during seed maturation. Here, we tested these hypotheses by studying PSV formation in Arabidopsis (Arabidopsis thaliana) embryos at different stages of seed maturation and recapitulated this process in Arabidopsis leaves reprogrammed to an embryogenic fate by inducing expression of the LEAFY COTYLEDON2 transcription factor. Confocal and immunoelectron microscopy indicated that both storage proteins and tonoplast proteins typical of PSV were delivered to the preexisting EV in embryos or to the lytic vacuole in reprogrammed leaf cells. In addition, sectioning through embryos at several developmental stages using serial block face scanning electron microscopy revealed the 3D architecture of forming PSV. Our results indicate that the preexisting EV is reprogrammed to become a PSV in Arabidopsis.
Texto completo:
1
Bases de dados:
MEDLINE
Assunto principal:
Sementes
/
Vacúolos
/
Arabidopsis
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Proteínas de Arabidopsis
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Proteínas de Armazenamento de Sementes
Idioma:
En
Revista:
Plant Physiol
Ano de publicação:
2018
Tipo de documento:
Article
País de afiliação:
Reino Unido