Thrombopoietin protects hematopoietic stem cells from retrotransposon-mediated damage by promoting an antiviral response.
J Exp Med
; 215(5): 1463-1480, 2018 05 07.
Article
em En
| MEDLINE
| ID: mdl-29615469
ABSTRACT
Maintenance of genomic integrity is crucial for the preservation of hematopoietic stem cell (HSC) potential. Retrotransposons, spreading in the genome through an RNA intermediate, have been associated with loss of self-renewal, aging, and DNA damage. However, their role in HSCs has not been addressed. Here, we show that mouse HSCs express various retroelements (REs), including long interspersed element-1 (L1) recent family members that further increase upon irradiation. Using mice expressing an engineered human L1 retrotransposition reporter cassette and reverse transcription inhibitors, we demonstrate that L1 retransposition occurs in vivo and is involved in irradiation-induced persistent γH2AX foci and HSC loss of function. Thus, RE represents an important intrinsic HSC threat. Furthermore, we show that RE activity is restrained by thrombopoietin, a critical HSC maintenance factor, through its ability to promote a potent interferon-like, antiviral gene response in HSCs. This uncovers a novel mechanism allowing HSCs to minimize irradiation-induced injury and reinforces the links between DNA damage, REs, and antiviral immunity.
Texto completo:
1
Bases de dados:
MEDLINE
Assunto principal:
Antivirais
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Trombopoetina
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Células-Tronco Hematopoéticas
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Retroelementos
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Citoproteção
Limite:
Animals
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Humans
Idioma:
En
Revista:
J Exp Med
Ano de publicação:
2018
Tipo de documento:
Article
País de afiliação:
França