Ashwagandha root extract exerts antiinflammatory effects in HaCaT cells by inhibiting the MAPK/NFκB pathways and by regulating cytokines.
Int J Mol Med
; 42(1): 425-434, 2018 Jul.
Article
em En
| MEDLINE
| ID: mdl-29620265
ABSTRACT
A paste composed of the boiled leaves and roots of the Ashwagandha plant is used to cure ulcer and swelling in Ayurvedic medicine. However, the effects of the hot water extract of Ashwagandha roots (ASHWEX), which is also used in Ayurveda, on skin have not been fully elucidated. Therefore, the present study investigated the antiinflammatory activity of ASHWEX on skin, by using the human keratinocyte cell line HaCaT. The results indicated that ASHWEX significantly inhibited mRNA expression of inflammatory cytokines, including interleukin (IL)8, IL6, tumor necrosis factor (TNFα), IL1ß and IL12, and promoted the mRNA expression of the antiinflammatory cytokine transforming growth factor (TGF)ß1 in HaCaT cells. In addition, ASHWEX inhibited the lipopolysaccharideinduced phosphorylation of p38 and cJun Nterminal kinase, as well as the nuclear translocation of nuclear factor (NF)κB p65. Downregulation of TNFα mRNA and upregulation of TGFß1 mRNA were also observed in vivo following ASHWEX treatment of mouse skin. In conclusion, the present study demonstrated that the antiinflammatory effect of ASHWEX may be due to its ability to suppress the NFκB and mitogenactivated protein kinase pathways, and to modulate cytokine expression. These results suggest that ASHWEX can potentially protect against skin inflammation.
Texto completo:
1
Bases de dados:
MEDLINE
Assunto principal:
Extratos Vegetais
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Queratinócitos
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Citocinas
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NF-kappa B
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Raízes de Plantas
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Sistema de Sinalização das MAP Quinases
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Anti-Inflamatórios
Limite:
Animals
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Humans
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Male
Idioma:
En
Revista:
Int J Mol Med
Assunto da revista:
BIOLOGIA MOLECULAR
/
GENETICA MEDICA
Ano de publicação:
2018
Tipo de documento:
Article
País de afiliação:
Japão