Chemically controlled aggregation of pluripotent stem cells.
Biotechnol Bioeng
; 115(8): 2061-2066, 2018 08.
Article
em En
| MEDLINE
| ID: mdl-29679475
ABSTRACT
Heterogeneity in pluripotent stem cell (PSC) aggregation leads to variability in mass transfer and signaling gradients between aggregates, which results in heterogeneous differentiation and therefore variability in product quality and yield. We have characterized a chemical-based method to control aggregate size within a specific, tunable range with low heterogeneity, thereby reducing process variability in PSC expansion. This method enables controlled, scalable, stirred suspension-based manufacturing of PSC cultures that are critical for the translation of regenerative medicine strategies to clinical products.
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Bases de dados:
MEDLINE
Assunto principal:
Biotecnologia
/
Agregação Celular
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Técnicas Citológicas
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Células-Tronco Pluripotentes
Idioma:
En
Revista:
Biotechnol Bioeng
Ano de publicação:
2018
Tipo de documento:
Article
País de afiliação:
Canadá