[Expression profiles of CaMKIIγ during osteoclast differentiation].
Zhong Nan Da Xue Xue Bao Yi Xue Ban
; 43(3): 240-245, 2018 Mar 28.
Article
em Zh
| MEDLINE
| ID: mdl-29701184
ABSTRACT
OBJECTIVE:
To study the expression profiles and the role of Ca2+/calmodulin-dependent protein kinase IIγ (CaMKIIγ) during osteoclast differentiation.â©Methods:
Mouse RAW264.7 cells were induced for osteoclastogenesis with 50 ng/mL receptor activator of nuclear factor-κB ligand (RANKL) and the cells were harvested at 0, 1, 3 and 5 days after induction. Tartrate-resistant acid phosphotase staining was performed to verify osteoclasts formation. RT-PCR, Western blot and immunofluorescent cytochemistry were used to detect the CaMKIIγ gene expression during osteoclastogenesis.â©Results:
The osteoclasts were formed at day 3 under RANKL induction and more osteoclasts were observed at day 5. At day 0, 1, 3 and 5, the relative level of CaMKIIγ mRNA were (1.067±0.179), (1.840±0.070), (9.493±0.453) and (30.767±0.573), respectively, and the relative protein level were (0.454±0.065), (0.613±0.021), (0.858±0.019) and (0.980±0.023), respectively. CaMKIIγ expression was increased in a time-dependent manner except relative protein level at day 1 (P<0.01), which showed no significant difference at day 0 (P>0.05). Immunofluorescence assay showed that CaMKIIγ protein was also increased with differentiation of osteoclasts.â©Conclusion:
The CaMKIIγ expression was increased in a time-depended manner during osteoclast differentiation and it might play a vital role during osteoclastogenesis.
Texto completo:
1
Bases de dados:
MEDLINE
Assunto principal:
Osteoclastos
/
Diferenciação Celular
/
Proteína Quinase Tipo 2 Dependente de Cálcio-Calmodulina
Limite:
Animals
Idioma:
Zh
Revista:
Zhong Nan Da Xue Xue Bao Yi Xue Ban
Assunto da revista:
MEDICINA
Ano de publicação:
2018
Tipo de documento:
Article
País de afiliação:
China