Facile Chemoenzymatic Synthesis of O-Mannosyl Glycans.
Angew Chem Int Ed Engl
; 57(30): 9268-9273, 2018 07 20.
Article
em En
| MEDLINE
| ID: mdl-29732660
ABSTRACT
Oâ
Mannosylation is a vital protein modification involved in brain and muscle development whereas the biological relevance of O-mannosyl glycans has remained largely unknown owing to the lack of structurally defined glycoforms. An efficient scaffold synthesis/enzymatic extension (SSEE) strategy was developed to prepare such structures by combining gram-scale convergent chemical syntheses of three scaffolds and strictly controlled sequential enzymatic extension catalyzed by glycosyltransferases. In total, 45 O-mannosyl glycans were obtained, covering the majority of identified mammalian structures. Subsequent glycan microarray analysis revealed fine specificities of glycan-binding proteins and specific antisera.
Palavras-chave
Texto completo:
1
Bases de dados:
MEDLINE
Assunto principal:
Polissacarídeos
/
Glicosiltransferases
/
Manose
Idioma:
En
Revista:
Angew Chem Int Ed Engl
Ano de publicação:
2018
Tipo de documento:
Article
País de afiliação:
Estados Unidos