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Mussel-inspired 3D fiber scaffolds for heart-on-a-chip toxicity studies of engineered nanomaterials.
Ahn, Seungkuk; Ardoña, Herdeline Ann M; Lind, Johan U; Eweje, Feyisayo; Kim, Sean L; Gonzalez, Grant M; Liu, Qihan; Zimmerman, John F; Pyrgiotakis, Georgios; Zhang, Zhenyuan; Beltran-Huarac, Juan; Carpinone, Paul; Moudgil, Brij M; Demokritou, Philip; Parker, Kevin Kit.
Afiliação
  • Ahn S; Disease Biophysics Group, Wyss Institute for Biologically Inspired Engineering, John A. Paulson School of Engineering and Applied Sciences, Harvard University, 29 Oxford Street, Cambridge, MA, 02138, USA.
  • Ardoña HAM; Disease Biophysics Group, Wyss Institute for Biologically Inspired Engineering, John A. Paulson School of Engineering and Applied Sciences, Harvard University, 29 Oxford Street, Cambridge, MA, 02138, USA.
  • Lind JU; Disease Biophysics Group, Wyss Institute for Biologically Inspired Engineering, John A. Paulson School of Engineering and Applied Sciences, Harvard University, 29 Oxford Street, Cambridge, MA, 02138, USA.
  • Eweje F; Department of Micro- and Nanotechnology, Technical University of Denmark, 2800, Kongens Lyngby, Denmark.
  • Kim SL; Disease Biophysics Group, Wyss Institute for Biologically Inspired Engineering, John A. Paulson School of Engineering and Applied Sciences, Harvard University, 29 Oxford Street, Cambridge, MA, 02138, USA.
  • Gonzalez GM; Disease Biophysics Group, Wyss Institute for Biologically Inspired Engineering, John A. Paulson School of Engineering and Applied Sciences, Harvard University, 29 Oxford Street, Cambridge, MA, 02138, USA.
  • Liu Q; Disease Biophysics Group, Wyss Institute for Biologically Inspired Engineering, John A. Paulson School of Engineering and Applied Sciences, Harvard University, 29 Oxford Street, Cambridge, MA, 02138, USA.
  • Zimmerman JF; Disease Biophysics Group, Wyss Institute for Biologically Inspired Engineering, John A. Paulson School of Engineering and Applied Sciences, Harvard University, 29 Oxford Street, Cambridge, MA, 02138, USA.
  • Pyrgiotakis G; Disease Biophysics Group, Wyss Institute for Biologically Inspired Engineering, John A. Paulson School of Engineering and Applied Sciences, Harvard University, 29 Oxford Street, Cambridge, MA, 02138, USA.
  • Zhang Z; Center for Nanotechnology and Nanotoxicology, Harvard T.H. Chan School of Public Health, Harvard University, Cambridge, MA, 02138, USA.
  • Beltran-Huarac J; Center for Nanotechnology and Nanotoxicology, Harvard T.H. Chan School of Public Health, Harvard University, Cambridge, MA, 02138, USA.
  • Carpinone P; Center for Nanotechnology and Nanotoxicology, Harvard T.H. Chan School of Public Health, Harvard University, Cambridge, MA, 02138, USA.
  • Moudgil BM; Center for Nanotechnology and Nanotoxicology, Harvard T.H. Chan School of Public Health, Harvard University, Cambridge, MA, 02138, USA.
  • Demokritou P; Center for Nanotechnology and Nanotoxicology, Harvard T.H. Chan School of Public Health, Harvard University, Cambridge, MA, 02138, USA.
  • Parker KK; Center for Nanotechnology and Nanotoxicology, Harvard T.H. Chan School of Public Health, Harvard University, Cambridge, MA, 02138, USA.
Anal Bioanal Chem ; 410(24): 6141-6154, 2018 Sep.
Article em En | MEDLINE | ID: mdl-29744562
ABSTRACT
Due to the unique physicochemical properties exhibited by materials with nanoscale dimensions, there is currently a continuous increase in the number of engineered nanomaterials (ENMs) used in consumer goods. However, several reports associate ENM exposure to negative health outcomes such as cardiovascular diseases. Therefore, understanding the pathological consequences of ENM exposure represents an important challenge, requiring model systems that can provide mechanistic insights across different levels of ENM-based toxicity. To achieve this, we developed a mussel-inspired 3D microphysiological system (MPS) to measure cardiac contractility in the presence of ENMs. While multiple cardiac MPS have been reported as alternatives to in vivo testing, most systems only partially recapitulate the native extracellular matrix (ECM) structure. Here, we show how adhesive and aligned polydopamine (PDA)/polycaprolactone (PCL) nanofiber can be used to emulate the 3D native ECM environment of the myocardium. Such nanofiber scaffolds can support the formation of anisotropic and contractile muscular tissues. By integrating these fibers in a cardiac MPS, we assessed the effects of TiO2 and Ag nanoparticles on the contractile function of cardiac tissues. We found that these ENMs decrease the contractile function of cardiac tissues through structural damage to tissue architecture. Furthermore, the MPS with embedded sensors herein presents a way to non-invasively monitor the effects of ENM on cardiac tissue contractility at different time points. These results demonstrate the utility of our MPS as an analytical platform for understanding the functional impacts of ENMs while providing a biomimetic microenvironment to in vitro cardiac tissue samples. Graphical Abstract Heart-on-a-chip integrated with mussel-inspired fiber scaffolds for a high-throughput toxicological assessment of engineered nanomaterials.
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Texto completo: 1 Bases de dados: MEDLINE Assunto principal: Bivalves / Nanoestruturas / Alicerces Teciduais / Dispositivos Lab-On-A-Chip / Nanofibras / Coração Limite: Animals Idioma: En Revista: Anal Bioanal Chem Ano de publicação: 2018 Tipo de documento: Article País de afiliação: Estados Unidos
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Bases de dados: MEDLINE Assunto principal: Bivalves / Nanoestruturas / Alicerces Teciduais / Dispositivos Lab-On-A-Chip / Nanofibras / Coração Limite: Animals Idioma: En Revista: Anal Bioanal Chem Ano de publicação: 2018 Tipo de documento: Article País de afiliação: Estados Unidos