Structure-based protein engineering of bacterial ß-xylosidase to increase the production yield of xylobiose from xylose.

Hong, Seokho; Kyung, Myungok; Jo, Inseong; Kim, Yong-Ro; Ha, Nam-Chul

Hong, Seokho; Kyung, Myungok; Jo, Inseong; Kim, Yong-Ro; Ha, Nam-Chul.

Afiliação

Hong S; Research Institute for Agriculture and Life Sciences, Center for Food and Bioconvergence, Center for Food Safety and Toxicology, Department of Agicultural Biotechnology, Seoul National University, Seoul, 08826, Republic of Korea.
Kyung M; Center for Food and Bioconvergence, Department of Biosystems & Biomaterials Science and Engineering, College of Agriculture and Life Sciences (CALS), Seoul National University, Seoul, 08826, Republic of Korea; R&D Center, TS Corporation, 116, Incheon, 22300, Republic of Korea.
Jo I; Research Institute for Agriculture and Life Sciences, Center for Food and Bioconvergence, Center for Food Safety and Toxicology, Department of Agicultural Biotechnology, Seoul National University, Seoul, 08826, Republic of Korea.
Kim YR; Center for Food and Bioconvergence, Department of Biosystems & Biomaterials Science and Engineering, College of Agriculture and Life Sciences (CALS), Seoul National University, Seoul, 08826, Republic of Korea.
Ha NC; Research Institute for Agriculture and Life Sciences, Center for Food and Bioconvergence, Center for Food Safety and Toxicology, Department of Agicultural Biotechnology, Seoul National University, Seoul, 08826, Republic of Korea. Electronic address: hanc210@snu.ac.kr.

Biochem Biophys Res Commun ; 501(3): 703-710, 2018 06 27.

Article em En | MEDLINE | ID: mdl-29752942

Assuntos

Bacillus/enzimologia; Dissacarídeos/metabolismo; Mutagênese Sítio-Dirigida/métodos; Xilose/metabolismo; Xilosidases/metabolismo; Bacillus/química; Bacillus/genética; Bacillus/metabolismo; Cristalografia por Raios X; Microbiologia Industrial/métodos; Modelos Moleculares; Conformação Proteica; Multimerização Proteica; Xilosidases/química; Xilosidases/genética

Palavras-chave

Bacillus pumilus; Crystal structure; Disaccharide production; Protein engineering; Xylobiose; ß-Xylosidase

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Texto completo: 1 Bases de dados: MEDLINE Assunto principal: Bacillus / Xilose / Xilosidases / Mutagênese Sítio-Dirigida / Dissacarídeos Idioma: En Revista: Biochem Biophys Res Commun Ano de publicação: 2018 Tipo de documento: Article

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