Electrospinning and wound healing activity of ß-chitin extracted from cuttlefish bone.
Carbohydr Polym
; 193: 205-211, 2018 Aug 01.
Article
em En
| MEDLINE
| ID: mdl-29773374
ABSTRACT
A nanofibrous ß-chitin web was fabricated via electrospinning for use as a novel wound dressing material. ß-chitin was extracted from cuttlefish bone using deproteinization and demineralization. First, cuttlefish bone was alkali-treated to remove the proteins and was then treated with the acid for demineralization. The extracted ß-chitin was dissolved in formic acid as solvent to evaluate its electrospinnability, and the electrospinnability increased remarkably when ß-chitin was blended with poly(ethylene oxide) (PEO) than without. The blended ß-chitin/PEO nanofibers had a fiber diameter of about 400â¯nm, and the diameter decreased after soaking in water to remove the PEO. The structural and physical properties of the ß-chitin material and its nanofibers were characterized using Attenuated total reflectance infrared spectroscopy (ATR-IR), Proton nuclear magnetic resonance (1H NMR), Scanning electron microscopy/Energy dispersive spectroscopy (SEM/EDS), X-ray diffraction (XRD), texturometry, viscometry and contact angle measurements, and an animal test was conducted to investigate the wound healing effect. The ß-chitin nanofibers were found to have great potential as nanomaterials for wound healing.
Palavras-chave
Texto completo:
1
Bases de dados:
MEDLINE
Assunto principal:
Cicatrização
/
Osso e Ossos
/
Quitina
Limite:
Animals
Idioma:
En
Revista:
Carbohydr Polym
Ano de publicação:
2018
Tipo de documento:
Article
País de afiliação:
Coréia do Sul