Distinct atrial natriuretic factor receptor sites on cultured bovine aortic smooth muscle and endothelial cells.
Biochem Biophys Res Commun
; 127(2): 433-42, 1985 Mar 15.
Article
em En
| MEDLINE
| ID: mdl-2983720
ABSTRACT
Cultured bovine aortic smooth muscle and endothelial cells each display distinct specific binding sites for radiolabeled atrial natriuretic peptide (ANF). 125I-pro-rANF (103-126)I binding to both cell types is rapid, reversible and competitive. Scatchard plots of the binding data show Bmax values of 5.5 and 0.1 - 2.1 X 10(5) sites/cell and Kd values of 2.1 and 0.3 nM for smooth muscle and endothelial cells, respectively. In addition, ANF elevates levels of cGMP substantially in both cell types at concentrations of ANF close to its Kd and Ki for binding. Sodium nitroprusside, however, has essentially no effect on cGMP levels in either cell type. These results show that distinct functionally active receptor sites for ANF exist on both vascular smooth muscle and endothelial cells.
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Bases de dados:
MEDLINE
Assunto principal:
Receptores de Superfície Celular
/
Endotélio
/
Músculo Liso Vascular
Limite:
Animals
Idioma:
En
Revista:
Biochem Biophys Res Commun
Ano de publicação:
1985
Tipo de documento:
Article