NMR assignments of the WBSCR27 protein related to Williams-Beuren syndrome.
Biomol NMR Assign
; 12(2): 303-308, 2018 10.
Article
em En
| MEDLINE
| ID: mdl-29868988
ABSTRACT
Williams-Beuren syndrome is a genetic disorder characterized by physiological and mental abnormalities, and is caused by hemizygous deletion of several genes in chromosome 7. One of the removed genes encodes the WBSCR27 protein. Bioinformatic analysis of the sequence of WBSCR27 indicates that it belongs to the family of SAM-dependent methyltransferases. However, exact cellular functions of this protein or phenotypic consequences of its deficiency are still unknown. Here we report nearly complete 1H, 15N, and 13C chemical shifts assignments of the 26 kDa WBSCR27 protein from Mus musculus in complex with the cofactor S-adenosyl-L-methionine (SAM). Analysis of the assigned chemical shifts allowed us to characterize the protein's secondary structure and backbone dynamics. The topology of the protein's fold confirms the assumption that the WBSCR27 protein belongs to the family of class I methyltransferases.
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Texto completo:
1
Bases de dados:
MEDLINE
Assunto principal:
Síndrome de Williams
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Ressonância Magnética Nuclear Biomolecular
Limite:
Animals
/
Humans
Idioma:
En
Revista:
Biomol NMR Assign
Assunto da revista:
BIOLOGIA MOLECULAR
/
MEDICINA NUCLEAR
Ano de publicação:
2018
Tipo de documento:
Article