In vivo imaging of the pathophysiological changes and neutrophil dynamics in influenza virus-infected mouse lungs.
Proc Natl Acad Sci U S A
; 115(28): E6622-E6629, 2018 07 10.
Article
em En
| MEDLINE
| ID: mdl-29941581
ABSTRACT
The pathophysiological changes that occur in lungs infected with influenza viruses are poorly understood. Here we established an in vivo imaging system that combines two-photon excitation microscopy and fluorescent influenza viruses of different pathogenicity. This approach allowed us to monitor and correlate several parameters and physiological changes including the spread of infection, pulmonary permeability, pulmonary perfusion speed, number of recruited neutrophils in infected lungs, and neutrophil motion in the lungs of live mice. Several physiological changes were larger and occurred earlier in mice infected with a highly pathogenic H5N1 influenza virus compared with those infected with a mouse-adapted human strain. These findings demonstrate the potential of our in vivo imaging system to provide novel information about the pathophysiological consequences of virus infections.
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Texto completo:
1
Bases de dados:
MEDLINE
Assunto principal:
Infecções por Orthomyxoviridae
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Microscopia de Fluorescência por Excitação Multifotônica
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Virus da Influenza A Subtipo H5N1
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Pulmão
Limite:
Animals
Idioma:
En
Revista:
Proc Natl Acad Sci U S A
Ano de publicação:
2018
Tipo de documento:
Article
País de afiliação:
Japão