[Generation of a new strain of NOD/SCID/IL2Rγ-/- mice with targeted disruption of Prkdc and IL2Rγ genes using CRISPR/Cas9 system].
Nan Fang Yi Ke Da Xue Xue Bao
; 38(6): 639-646, 2018 Jun 20.
Article
em Zh
| MEDLINE
| ID: mdl-29997084
OBJECTIVE: The NOD/SCID/IL2Rγ- /- (NSG) mouse strain is the most widely used immunodeficient strain for xenograft transplantation. However, the existing SCID mutation is a spontaneous mutation of the Prkdc gene, which leads to leaky T cell developmental block and difficulty in genotyping. It is therefore important to develop a new strain of NSG mice with targeted disruption of Prkdc and IL2Rγ genes. METHODS: Targeted disruption of Prkdc and IL2Rγ genes was achieved using the CRISPR/Cas9 system. By intercrossing the knockout and NOD mice, we obtained a novel strain of NOD/SCID/IL2Rγ- /-(NSG) mice, denoted as cNSG (Chinese NSG) mice. RESULTS: In addition to the NOD mutation, cNSG mice exhibited a complete absence of T cells, B cells and NK cells. cNSG mice allowed more efficient engraftment of human cancer cells than the commonly used immunodeficient nude mice. CONCLUSION: cNSG mice will provide an important xenotransplantation model for biomedical research.
Texto completo:
1
Bases de dados:
MEDLINE
Assunto principal:
Transplante Heterólogo
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Proteínas Nucleares
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Camundongos SCID
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Camundongos Endogâmicos NOD
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Proteínas de Ligação a DNA
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Proteína Quinase Ativada por DNA
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Subunidade gama Comum de Receptores de Interleucina
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Sistemas CRISPR-Cas
Limite:
Animals
Idioma:
Zh
Revista:
Nan Fang Yi Ke Da Xue Xue Bao
Ano de publicação:
2018
Tipo de documento:
Article