Why the COI barcode should be the community DNA metabarcode for the metazoa.
Mol Ecol
; 27(20): 3968-3975, 2018 10.
Article
em En
| MEDLINE
| ID: mdl-30129071
ABSTRACT
Metabarcoding of complex metazoan communities is increasingly being used to measure biodiversity in terrestrial, freshwater and marine ecosystems, revolutionizing our ability to observe patterns and infer processes regarding the origin and conservation of biodiversity. A fundamentally important question is which genetic marker to amplify, and although the mitochondrial cytochrome oxidase subunit I (COI) gene is one of the more widely used markers in metabarcoding for the Metazoa, doubts have recently been raised about its suitability. We argue that (a) the extensive coverage of reference sequence databases for COI; (b) the variation it presents; (c) the comparative advantages for denoising protein-coding genes; and (d) recent advances in DNA sequencing protocols argue in favour of standardizing for the use of COI for metazoan community samples. We also highlight where research efforts should focus to maximize the utility of metabarcoding.
Palavras-chave
Texto completo:
1
Bases de dados:
MEDLINE
Assunto principal:
Código de Barras de DNA Taxonômico
Limite:
Animals
Idioma:
En
Revista:
Mol Ecol
Assunto da revista:
BIOLOGIA MOLECULAR
/
SAUDE AMBIENTAL
Ano de publicação:
2018
Tipo de documento:
Article
País de afiliação:
Espanha