Description and initial characterization of metatranscriptomic nidovirus-like genomes from the proposed new family Abyssoviridae, and from a sister group to the Coronavirinae, the proposed genus Alphaletovirus.
Virology
; 524: 160-171, 2018 11.
Article
em En
| MEDLINE
| ID: mdl-30199753
ABSTRACT
Transcriptomics has the potential to discover new RNA virus genomes by sequencing total intracellular RNA pools. In this study, we have searched publicly available transcriptomes for sequences similar to viruses of the Nidovirales order. We report two potential nidovirus genomes, a highly divergent 35.9â¯kb likely complete genome from the California sea hare Aplysia californica, which we assign to a nidovirus named Aplysia abyssovirus 1 (AAbV), and a coronavirus-like 22.3â¯kb partial genome from the ornamented pygmy frog Microhyla fissipes, which we assign to a nidovirus named Microhyla alphaletovirus 1 (MLeV). AAbV was shown to encode a functional main proteinase, and a translational readthrough signal. Phylogenetic analysis suggested that AAbV represents a new family, proposed here as Abyssoviridae. MLeV represents a sister group to the other known coronaviruses. The importance of MLeV and AAbV for understanding nidovirus evolution, and the origin of terrestrial nidoviruses are discussed.
Palavras-chave
Texto completo:
1
Bases de dados:
MEDLINE
Assunto principal:
Genoma Viral
/
Coronaviridae
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Nidovirales
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Infecções por Nidovirales
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Transcriptoma
País/Região como assunto:
America do norte
Idioma:
En
Revista:
Virology
Ano de publicação:
2018
Tipo de documento:
Article
País de afiliação:
Reino Unido