Recovering cellular biomass from fluids using chemical flocculation.
Environ Microbiol Rep
; 10(6): 686-694, 2018 12.
Article
em En
| MEDLINE
| ID: mdl-30230256
ABSTRACT
We developed an efficient, scalable and inexpensive method for recovering cellular biomass from complex fluid matrices that cannot be processed using conventional filtration methods. The method uses chemical flocculation with iron oxyhydroxides, is capable of recovering greater than 90% of cellular biomass from fluids with more than 103 cells ml-1 , and was validated using both mock communities and field samples. High quality DNA can be readily extracted from iron flocs using standard soil extraction kits. We applied chemical flocculation to fracing fluids from British Columbia, Canada and recovered a diversity of microbial taxa including abundant members of the Epsilon- and Deltaproteobacteria previously recovered from shale gas operations in the United States. Application of chemical flocculation presents new opportunities for scalable time-series monitoring and experimentation on complex fluid matrices including microbial community profiling and shotgun metagenomics over gas production well completion cycles.
Texto completo:
1
Bases de dados:
MEDLINE
Assunto principal:
Microbiologia da Água
/
DNA Bacteriano
/
Técnicas Microbiológicas
/
Biomassa
Idioma:
En
Revista:
Environ Microbiol Rep
Ano de publicação:
2018
Tipo de documento:
Article
País de afiliação:
Canadá