Paenibacillus paeoniae sp. nov., a novel endophytic bacterium isolated from leaf of Paeonia lactiflora Pall.
Int J Syst Evol Microbiol
; 68(11): 3606-3610, 2018 Nov.
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em En
| MEDLINE
| ID: mdl-30252640
ABSTRACT
A Gram-stain-positive, aerobic, rod-shaped, endospore-forming bacterium, designated strain M4BSY-1T, was originally isolated from a surface-sterilized leaf of Paeonia lactiflora Pall. in Guizhou, China. The bacterium was characterized by a polyphasic approach to determine its taxonomic position. 16S rRNA gene sequence comparison revealed that strain M4BSY-1T belongs to the genus Paenibacillus and most closely to Paenibacilluspinioli NB5T (98.31â% similarity). Neither substrate nor aerial mycelia formed, and no diffusible pigments were observed on the media tested. Strain M4BSY-1T grew in the pH range 7.0-13.0 (optimum, 7.0-8.0), at temperatures between 10-37 °C (optimum, 30 °C) and at 0-5â% (w/v) NaCl (optimum, 1-2â%). The predominant menaquinone was MK-7. The major fatty acids were anteiso-C15ââ0, C16ââ0 and iso-C16ââ0. The polar lipids comprised diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, two unidentified unknown aminophospholipids and three unidentified phospholipids. The DNA G+C content was 48.8 mol%. According to the phylogenetic, phenotypic and chemotaxonomic evidence, strain M4BSY-1T was clearly distinguishable from other species with validly published names in the genus Paenibacillus and should therefore be classified as a novel species, and we suggest the name Paenibacillus paeoniae sp. nov. The type strain is M4BSY-1T (=KCTC 33997T=CGMCC 1.13667T).
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Bases de dados:
MEDLINE
Assunto principal:
Filogenia
/
Folhas de Planta
/
Paeonia
/
Paenibacillus
País/Região como assunto:
Asia
Idioma:
En
Revista:
Int J Syst Evol Microbiol
Assunto da revista:
MICROBIOLOGIA
Ano de publicação:
2018
Tipo de documento:
Article