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Molecular characterization of human adenovirus associated with acute respiratory infections in Cameroon from 2011 to 2014.
Kenmoe, Sebastien; Vernet, Marie-Astrid; Le Goff, Jerôme; Penlap, Véronique Beng; Vabret, Astrid; Njouom, Richard.
Afiliação
  • Kenmoe S; Virology Department, "Centre Pasteur du Cameroun", P.O.Box 1274, Yaounde, Cameroon.
  • Vernet MA; Département de Biochimie, Université de Yaoundé 1, BP 812, Yaounde, Cameroon.
  • Le Goff J; Normandie Université, 14032, Caen, France.
  • Penlap VB; UNICAEN, UNIROUEN, GRAM, 14000, Caen, France.
  • Vabret A; University Hospital of Caen, Department of Virology, 14000, Caen, France.
  • Njouom R; Virology Department, "Centre Pasteur du Cameroun", P.O.Box 1274, Yaounde, Cameroon.
Virol J ; 15(1): 153, 2018 10 03.
Article em En | MEDLINE | ID: mdl-30285778
ABSTRACT

BACKGROUND:

Human adenoviruses (HAdVs) cause a wide range of diseases worldwide, including respiratory infections. Studies on HAdV molecular epidemiology are limited in Cameroon. The purpose of this study is to document the different types HAdV circulating in Cameroon in children with acute respiratory infections.

METHODS:

Nasopharyngeal swabs were collected from 811 children under 15 years from 2011 to 2014. The HAdV detection was assessed by semi-quantitative generic PCR r-gene®. The HAdV-positive samples were typed by amplification and sequencing of partial hexon gene and a real-time PCR. Demographic data were collected and analyzed. The infection and hospitalization risk factors were assessed thought the Chi-square test.

RESULTS:

A total of 137/220 HAdV-positive samples were amplified successfully. Six species of HAdV (Mastadenovirus A to F) were detected with B (108/220) and C (47/220) being the predominant strains. Hospitalization and age were significantly associated to HAdV-B and HAdV-C respectively. Phylogenetic analysis of HAdV-B3 virus (18) and B7 (5) shows a conserved and a significant temporal stability in relation to the reference sequence (99.1 to 100% of similarity).

CONCLUSION:

This study reported HAdV species and types detected in children with acute respiratory infections in Cameroon between September 2011 and July 2014. These results support further evaluation of the spatio-temporal circulation pattern of HAdV species and types in Cameroon.
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Texto completo: 1 Bases de dados: MEDLINE Assunto principal: Infecções Respiratórias / Variação Genética / Adenovírus Humanos / Infecções por Adenoviridae Tipo de estudo: Etiology_studies / Risk_factors_studies Limite: Adolescent / Child / Child, preschool / Female / Humans / Infant / Male / Newborn País/Região como assunto: Africa Idioma: En Revista: Virol J Assunto da revista: VIROLOGIA Ano de publicação: 2018 Tipo de documento: Article País de afiliação: Camarões

Texto completo: 1 Bases de dados: MEDLINE Assunto principal: Infecções Respiratórias / Variação Genética / Adenovírus Humanos / Infecções por Adenoviridae Tipo de estudo: Etiology_studies / Risk_factors_studies Limite: Adolescent / Child / Child, preschool / Female / Humans / Infant / Male / Newborn País/Região como assunto: Africa Idioma: En Revista: Virol J Assunto da revista: VIROLOGIA Ano de publicação: 2018 Tipo de documento: Article País de afiliação: Camarões