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Lentiviral transduction of mammalian cells for fast, scalable and high-level production of soluble and membrane proteins.
Elegheert, Jonathan; Behiels, Ester; Bishop, Benjamin; Scott, Suzanne; Woolley, Rachel E; Griffiths, Samuel C; Byrne, Eamon F X; Chang, Veronica T; Stuart, David I; Jones, E Yvonne; Siebold, Christian; Aricescu, A Radu.
Afiliação
  • Elegheert J; Division of Structural Biology, Wellcome Centre for Human Genetics, University of Oxford, Oxford, UK. jonathan.elegheert@u-bordeaux.fr.
  • Behiels E; Interdisciplinary Institute for Neuroscience, University of Bordeaux, Bordeaux, France. jonathan.elegheert@u-bordeaux.fr.
  • Bishop B; Interdisciplinary Institute for Neuroscience, CNRS UMR 5297, Bordeaux, France. jonathan.elegheert@u-bordeaux.fr.
  • Scott S; Division of Structural Biology, Wellcome Centre for Human Genetics, University of Oxford, Oxford, UK.
  • Woolley RE; Interdisciplinary Institute for Neuroscience, University of Bordeaux, Bordeaux, France.
  • Griffiths SC; Interdisciplinary Institute for Neuroscience, CNRS UMR 5297, Bordeaux, France.
  • Byrne EFX; Division of Structural Biology, Wellcome Centre for Human Genetics, University of Oxford, Oxford, UK.
  • Chang VT; Division of Structural Biology, Wellcome Centre for Human Genetics, University of Oxford, Oxford, UK.
  • Stuart DI; MRC Laboratory of Molecular Biology, Cambridge Biomedical Campus, Cambridge, UK.
  • Jones EY; Division of Structural Biology, Wellcome Centre for Human Genetics, University of Oxford, Oxford, UK.
  • Siebold C; Division of Structural Biology, Wellcome Centre for Human Genetics, University of Oxford, Oxford, UK.
  • Aricescu AR; Division of Structural Biology, Wellcome Centre for Human Genetics, University of Oxford, Oxford, UK.
Nat Protoc ; 13(12): 2991-3017, 2018 12.
Article em En | MEDLINE | ID: mdl-30455477

Texto completo: 1 Bases de dados: MEDLINE Assunto principal: Plasmídeos / Transdução Genética / Lentivirus / Proteínas de Membrana Limite: Humans Idioma: En Revista: Nat Protoc Ano de publicação: 2018 Tipo de documento: Article País de afiliação: Reino Unido

Texto completo: 1 Bases de dados: MEDLINE Assunto principal: Plasmídeos / Transdução Genética / Lentivirus / Proteínas de Membrana Limite: Humans Idioma: En Revista: Nat Protoc Ano de publicação: 2018 Tipo de documento: Article País de afiliação: Reino Unido