Hydrogen peroxide-mediated oxidative stress induces inflammasome activation in term human placental explants.
Pregnancy Hypertens
; 14: 29-36, 2018 Oct.
Article
em En
| MEDLINE
| ID: mdl-30527115
ABSTRACT
BACKGROUND:
The placenta is a multifunctional organ that can suffer with imbalances between pro- and antioxidant molecules, contributing for inflammatory imbalance. The inflammation generated by oxidative stress may induce inflammasome activation, an essential complex for pro-inflammatory cytokine production.OBJECTIVE:
The aim of this study was to evaluate whether hydrogen peroxide (H2O2) mediated oxidative stress induces inflammasome activation on placental explants. STUDYDESIGN:
Tissue cultures of placental explants obtained from normotensive pregnant women were performed in different concentrations of H2O2. Gene expressions of NLRP3, caspase-1, IL-1ß, TNF-α and IL-10 were evaluated by qPCR. Superoxide dismutase (SOD), catalase, Heat shock protein 70 (Hsp70), Caspase-1, TNF-α, IL-1ß, IL-10 and human Chorionic Gonadotropin (hCG) were determined by ELISA.RESULTS:
Concentrations of catalase, Hsp70, hCG and SOD were higher in cultures with 100 and 1000⯵M H2O2 compared to controls. Gene and protein expressions of TNF-α and IL-1ß were elevated in cultures with 1000⯵M H2O2 compared to controls. This concentration led to inflammasome activation, by increasing gene expressions of NLRP3, caspase-1 and IL-1ß. In contrast, gene and protein expressions of IL-10 were reduced at 100 and 1000⯵M H2O2. Protein expression of caspase-1 was higher in cultures of 100⯵M H2O2 compared to controls. Treatment with Glybenclamide at 200⯵M was used to prevent NLRP3 inflammasome activation. This concentration reduced protein expression of caspase-1 compared to culture with only H2O2 and control cultures.CONCLUSIONS:
Our results confirm that H2O2 induces oxidative stress on placental explants and demonstrate that cell responses to this stress involve inflammasome activation.Palavras-chave
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Bases de dados:
MEDLINE
Assunto principal:
Placenta
/
Pré-Eclâmpsia
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Inflamassomos
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Peróxido de Hidrogênio
Limite:
Female
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Humans
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Pregnancy
Idioma:
En
Revista:
Pregnancy Hypertens
Ano de publicação:
2018
Tipo de documento:
Article