Toxico-pathological effects of meglumine antimoniate on human umbilical vein endothelial cells.
Toxicol In Vitro
; 56: 10-18, 2019 Apr.
Article
em En
| MEDLINE
| ID: mdl-30599190
ABSTRACT
Leishmaniasis is one of the most important parasitic diseases after malaria. The standard treatment of leishmaniasis includes pentavalent antimonials (SbV); however, these drugs are associated with serious adverse effects. There have been very few studies pertaining to their side effects and mechanism of action in the fetus. This investigation examines the effects of meglumine antimoniate (MA) on the survival rate, angiogenesis and cellular apoptosis in the human umbilical vein endothelial cells (HUVECs). HUVECs were treated with varying doses of MA (100-800⯵g/ml) for 24, 48 and 72â¯h and the survival rate was studied by colorimetric assay, flow cytometry, immunocytochemistry, migration (scratch) assay and tube formation assay. The results of quantitative real-time PCR (qPCR) studies indicated that the most important genes involved in presenting angiogenesis included VEGF and its receptors (Kdr and Flt-1), NP1 and Hif-1α genes including the anti-apoptotic gene of Bcl2, were significantly reduced compared to the control group (pâ¯<â¯0.05). In contrast, the most leading genes involved in the phenomenon of apoptosis were P53, Bax, Bak, Apaf-1 and caspases 3, 8 and 9, which were significantly up regulated compared to the control group (pâ¯<â¯0.05).
Palavras-chave
Texto completo:
1
Bases de dados:
MEDLINE
Assunto principal:
Células Endoteliais da Veia Umbilical Humana
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Antimoniato de Meglumina
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Antiprotozoários
Limite:
Humans
Idioma:
En
Revista:
Toxicol In Vitro
Assunto da revista:
TOXICOLOGIA
Ano de publicação:
2019
Tipo de documento:
Article
País de afiliação:
Irã