A Hybrid Approach for Protein Structure Determination Combining Sparse NMR with Evolutionary Coupling Sequence Data.
Adv Exp Med Biol
; 1105: 153-169, 2018.
Article
em En
| MEDLINE
| ID: mdl-30617828
ABSTRACT
While 3D structure determination of small (<15 kDa) proteins by solution NMR is largely automated and routine, structural analysis of larger proteins is more challenging. An emerging hybrid strategy for modeling protein structures combines sparse NMR data that can be obtained for larger proteins with sequence co-variation data, called evolutionary couplings (ECs), obtained from multiple sequence alignments of protein families. This hybrid "EC-NMR" method can be used to accurately model larger (15-60 kDa) proteins, and more rapidly determine structures of smaller (5-15 kDa) proteins using only backbone NMR data. The resulting structures have accuracies relative to reference structures comparable to those obtained with full backbone and sidechain NMR resonance assignments. The requirement that evolutionary couplings (ECs) are consistent with NMR data recorded on a specific member of a protein family, under specific conditions, potentially also allows identification of ECs that reflect alternative allosteric or excited states of the protein structure.
Palavras-chave
Texto completo:
1
Bases de dados:
MEDLINE
Assunto principal:
Conformação Proteica
/
Proteínas
/
Evolução Molecular
/
Ressonância Magnética Nuclear Biomolecular
Idioma:
En
Revista:
Adv Exp Med Biol
Ano de publicação:
2018
Tipo de documento:
Article
País de afiliação:
Estados Unidos