Increased Hematopoietic Extracellular RNAs and Vesicles in the Lung during Allergic Airway Responses.
Cell Rep
; 26(4): 933-944.e4, 2019 01 22.
Article
em En
| MEDLINE
| ID: mdl-30673615
ABSTRACT
Extracellular RNAs (exRNAs) can be released by numerous cell types in vitro, are often protected within vesicles, and can modify recipient cell function. To determine how the composition and cellular sources of exRNAs and the extracellular vesicles (EVs) that carry them change in vivo during tissue inflammation, we analyzed bronchoalveolar lavage fluid (BALF) from mice before and after lung allergen challenge. In the lung, extracellular microRNAs (ex-miRNAs) had a composition that was highly correlated with airway-lining epithelium. Using cell type-specific membrane tagging and single vesicle flow, we also found that 80% of detected vesicles were of epithelial origin. After the induction of allergic airway inflammation, miRNAs selectively expressed by immune cells, including miR-223 and miR-142a, increased and hematopoietic-cell-derived EVs also increased >2-fold. These data demonstrate that infiltrating immune cells release ex-miRNAs and EVs in inflamed tissues to alter the local extracellular environment.
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Texto completo:
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Bases de dados:
MEDLINE
Assunto principal:
Asma
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Líquido da Lavagem Broncoalveolar
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MicroRNAs
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Pulmão
Limite:
Animals
Idioma:
En
Revista:
Cell Rep
Ano de publicação:
2019
Tipo de documento:
Article
País de afiliação:
Namíbia