Guide RNAs with embedded barcodes boost CRISPR-pooled screens.
Genome Biol
; 20(1): 20, 2019 01 24.
Article
em En
| MEDLINE
| ID: mdl-30678704
ABSTRACT
We report a new method using re-designed guide RNAs with internal barcodes (iBARs) embedded in their loop regions. Our iBAR approach outperforms the conventional method by producing screening results with much lower false-positive and false-negative rates especially with a high multiplicity of infection (MOI). Importantly, the iBAR approach reduces the starting cells at high MOI significantly with greatly improved efficiency and accuracy compared with the canonical CRISPR screens at a low MOI. This new system is particularly useful when the source of cells is limited or when it is difficult to control viral infection for in vivo screening.
Texto completo:
1
Bases de dados:
MEDLINE
Assunto principal:
RNA Guia de Cinetoplastídeos
/
Genômica
/
Sistemas CRISPR-Cas
Tipo de estudo:
Evaluation_studies
Limite:
Humans
Idioma:
En
Revista:
Genome Biol
Assunto da revista:
BIOLOGIA MOLECULAR
/
GENETICA
Ano de publicação:
2019
Tipo de documento:
Article