miR-33 and RIP140 participate in LPS-induced acute lung injury
Turk J Med Sci
; 49(1): 422-428, 2019 Feb 11.
Article
em En
| MEDLINE
| ID: mdl-30761836
ABSTRACT
Background/aim:
Pulmonary microvascular endothelial cells (PMVECs) play a pivotal role in the process of acute lung injury (ALI), which can be induced by lipopolysaccharide (LPS). Numerous reports have indicated that both miR-33 and RIP140 are involved in the inflammatory response in macrophages. In this study, we sought to investigate whether miR-33 and RIP140 participate in ALI induced by LPS. Materials andmethods:
First, we isolated and identified PMVECs from BALB/c mice. Subsequently, both PMVECs and BALB/c mice were treated with PBS, LPS, or pyrrolidine dithiocarbamate (PDTC) plus LPS and divided into three groups control (PBS), LPS (LPS), and L+P (LPS plus PDTC) groups. We assessed pathology by hematoxylin and eosin staining, and miR-33 and RIP140 expression levels were examined using quantitative PCR and Western blot analyses.Results:
Our results demonstrated that LPS can induce PMVEC injury and ALI and that LPS treatment significantly decreased miR-33 expression compared with controls (P < 0.001, n = 5). On the contrary, RIP140 was markedly overexpressed by LPS treatment (P < 0.001, n = 5). However, this alteration can be inhibited by pretreatment with PDTC before LPS (P < 0.05, n = 5).Conclusion:
This study is the first to confirm that both miR-33 and RIP140 participate in LPS-induced PMVEC injury and ALI, which may help uncover the mechanism of ALIPalavras-chave
Texto completo:
1
Bases de dados:
MEDLINE
Assunto principal:
MicroRNAs
/
Lesão Pulmonar Aguda
/
Proteína 1 de Interação com Receptor Nuclear
Limite:
Animals
Idioma:
En
Revista:
Turk J Med Sci
Ano de publicação:
2019
Tipo de documento:
Article