Your browser doesn't support javascript.
loading
Effectiveness of umbilical cord mesenchymal stem cells in patients with critical limb ischemia.
Gao, Wen-Hui; Gao, Hong-Ye; Li, Yue-Tong; Huang, Ping-Ping.
Afiliação
  • Gao WH; Institute of Hematology, General Medical Center, Blood Diseases Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Tianjin, China.
  • Gao HY; Institute of Hematology, General Medical Center, Blood Diseases Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Tianjin, China.
  • Li YT; Tianjin University of Traditional Chinese Medicine, Tianjin, China.
  • Huang PP; Institute of Hematology, General Medical Center, Blood Diseases Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Tianjin, China. Electronic address: huangpp@ihcams.ac.cn.
Med Clin (Barc) ; 153(9): 341-346, 2019 11 15.
Article em En, Es | MEDLINE | ID: mdl-30926157
ABSTRACT
INTRODUCTION AND

OBJECTIVE:

Transplantation of umbilical cord mesenchymal stem cells (UC-MSCs) has been shown to be effective in treating critical limb ischemia (CLI). However, the mechanism of MSCs-mediated improvements, especially on the immune-inflammatory aspects of this disease, is still unknown. In this study, we investigated the changes in T-lymphocyte subpopulations and inflammatory mediators (such as IL-6, IL-10 and TNF-α) in PBMCs from CLI patients after UC-MSCs treatment and correlation between inflammatory mediators and EPCs. PATIENTS AND

METHODS:

8 patients received UC-MSCs transplantation. Before the treatment, at 24h and 1 month thereafter, peripheral blood samples were collected from 8 patients and 8 healthy volunteers. Patients were evaluated for changes in IL-6, IL-10, TNF-α and levels of circulating EPCs.

RESULTS:

TNF-α and IL-6 serum levels increased at 24h (p=0.017, p=0.099) after treatment and then decreased at 1 month (p=0.031, p=0.072) compared with those before treatment. The percentages of CD3+T, CD3+CD4+T-lymphocytes and NK cells decreased significantly after UC-MSCs treatment (p=0.002, p=0.012 and p=0.029, respectively). TNF-α (r=-0.602, p=0.038) was shown to be inversely correlated with the number of circulating EPCs.

CONCLUSIONS:

This study demonstrates that UC-MSCs have anti-inflammatory and immunomodulation properties in CLI and suggests that UC-MSCs promote healing of non-healing wounds.
Assuntos
Palavras-chave

Texto completo: 1 Bases de dados: MEDLINE Assunto principal: Transplante de Células-Tronco Mesenquimais / Extremidades / Isquemia Limite: Adult / Aged / Female / Humans / Male / Middle aged Idioma: En / Es Revista: Med Clin (Barc) Ano de publicação: 2019 Tipo de documento: Article País de afiliação: China

Texto completo: 1 Bases de dados: MEDLINE Assunto principal: Transplante de Células-Tronco Mesenquimais / Extremidades / Isquemia Limite: Adult / Aged / Female / Humans / Male / Middle aged Idioma: En / Es Revista: Med Clin (Barc) Ano de publicação: 2019 Tipo de documento: Article País de afiliação: China