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Quantitative assessment of myocardial blood flow and extracellular volume fraction using 68Ga-DOTA-PET: A feasibility and validation study in large animals.
Velasco, Carlos; Mota-Cobián, Adriana; Mota, Rubén A; Pellico, Juan; Herranz, Fernando; Galán-Arriola, Carlos; Ibáñez, Borja; Ruiz-Cabello, Jesús; Mateo, Jesús; España, Samuel.
Afiliação
  • Velasco C; Centro Nacional de Investigaciones Cardiovasculares (CNIC), Melchor Fernández Almagro, 3, 28029, Madrid, Spain.
  • Mota-Cobián A; Universidad Complutense de Madrid, Madrid, Spain.
  • Mota RA; Centro Nacional de Investigaciones Cardiovasculares (CNIC), Melchor Fernández Almagro, 3, 28029, Madrid, Spain.
  • Pellico J; Universidad Complutense de Madrid, Madrid, Spain.
  • Herranz F; Centro Nacional de Investigaciones Cardiovasculares (CNIC), Melchor Fernández Almagro, 3, 28029, Madrid, Spain.
  • Galán-Arriola C; Charles River Laboratories España, Cerdanyola, Spain.
  • Ibáñez B; Centro Nacional de Investigaciones Cardiovasculares (CNIC), Melchor Fernández Almagro, 3, 28029, Madrid, Spain.
  • Ruiz-Cabello J; CIBER de Enfermedades Respiratorias, Madrid, Spain.
  • Mateo J; Centro Nacional de Investigaciones Cardiovasculares (CNIC), Melchor Fernández Almagro, 3, 28029, Madrid, Spain.
  • España S; CIBER de Enfermedades Respiratorias, Madrid, Spain.
J Nucl Cardiol ; 27(4): 1249-1260, 2020 08.
Article em En | MEDLINE | ID: mdl-30927149
BACKGROUND: Here we evaluated the feasibility of PET with Gallium-68 (68Ga)-labeled DOTA for non-invasive assessment of myocardial blood flow (MBF) and extracellular volume fraction (ECV) in a pig model of myocardial infarction. We also aimed to validate MBF measurements using microspheres as a gold standard in healthy pigs. METHODS: 8 healthy pigs underwent three sequential 68Ga-DOTA-PET/CT scans at rest and during pharmacological stress with simultaneous injection of fluorescent microspheres to validate MBF measurements. Myocardial infarction was induced in 5 additional pigs, which underwent 68Ga-DOTA-PET/CT examinations 7-days after reperfusion. Dynamic PET images were reconstructed and fitted to obtain MBF and ECV parametric maps. RESULTS: MBF assessed with 68Ga-DOTA-PET showed good correlation (y = 0.96x + 0.11, r = 0.91) with that measured with microspheres. MBF values obtained with 68Ga-DOTA-PET in the infarcted area (LAD, left anterior descendant) were significantly reduced in comparison to remote ones LCX (left circumflex artery, P < 0.0001) and RCA (right coronary artery, P < 0.0001). ECV increased in the infarcted area (P < 0.0001). CONCLUSION: 68Ga-DOTA-PET allowed non-invasive assessment of MBF and ECV in pigs with myocardial infarction and under rest-stress conditions. This technique could provide wide access to quantitative measurement of both MBF and ECV with PET imaging.
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Texto completo: 1 Bases de dados: MEDLINE Assunto principal: Compostos Radiofarmacêuticos / Circulação Coronária / Tomografia por Emissão de Pósitrons / Radioisótopos de Gálio / Compostos Heterocíclicos com 1 Anel / Infarto do Miocárdio Limite: Animals Idioma: En Revista: J Nucl Cardiol Assunto da revista: CARDIOLOGIA Ano de publicação: 2020 Tipo de documento: Article País de afiliação: Espanha

Texto completo: 1 Bases de dados: MEDLINE Assunto principal: Compostos Radiofarmacêuticos / Circulação Coronária / Tomografia por Emissão de Pósitrons / Radioisótopos de Gálio / Compostos Heterocíclicos com 1 Anel / Infarto do Miocárdio Limite: Animals Idioma: En Revista: J Nucl Cardiol Assunto da revista: CARDIOLOGIA Ano de publicação: 2020 Tipo de documento: Article País de afiliação: Espanha