Supramaximal Intensity Hypoxic Exercise and Vascular Function Assessment in Mice.
J Vis Exp
; (145)2019 03 15.
Article
em En
| MEDLINE
| ID: mdl-30933059
ABSTRACT
Exercise training is an important strategy for maintaining health and preventing many chronic diseases. It is the first line of treatment recommended by international guidelines for patients suffering from cardiovascular diseases, more specifically, lower extremity artery diseases, where the patients' walking capacity is considerably altered, affecting their quality of life. Traditionally, both low continuous exercise and interval training have been used. Recently, supramaximal training has also been shown to improve athletes' performances via vascular adaptations, amongst other mechanisms. The combination of this type of training with hypoxia could bring an additional and/or synergic effect, which could be of interest for certain pathologies. Here, we describe how to perform supramaximal intensity training sessions in hypoxia on healthy mice at 150% of their maximal speed, using a motorized treadmill and a hypoxic box. We also show how to dissect the mouse in order to retrieve organs of interest, particularly the pulmonary artery, the abdominal aorta, and the iliac artery. Finally, we show how to perform ex vivo vascular function assessment on the retrieved vessels, using isometric tension studies.
Texto completo:
1
Bases de dados:
MEDLINE
Assunto principal:
Condicionamento Físico Animal
/
Vasos Sanguíneos
/
Hipóxia
Tipo de estudo:
Guideline
Limite:
Animals
Idioma:
En
Revista:
J Vis Exp
Ano de publicação:
2019
Tipo de documento:
Article