Lack of IκBNS promotes cholate-containing high-fat diet-induced inflammation and atherogenesis in low-density lipoprotein (LDL) receptor-deficient mice.
Int J Cardiol Heart Vasc
; 23: 100344, 2019 Jun.
Article
em En
| MEDLINE
| ID: mdl-30976650
ABSTRACT
BACKGROUND:
IκBNS, a nuclear IκB protein, regulates a subset of Toll-like receptor (TLR) dependent genes. A cholate-containing high-fat diet (HFD(CA(+))) induces TLR4 mediated early inflammatory response. The present study aims to clarify that the lack of IκBNS promotes atherogenesis in low-density lipoprotein receptor-deficient (LDLr-/-) mice fed HFD(CA(+)) compared with those fed a cholate-free HFD (HFD(CA(-))). METHODS ANDRESULTS:
Mice that lacked IκBNS (IκBNS-/-) were crossed with LDLr-/- mice and formation of atherosclerotic lesions was analyzed after 6-week consumption of HFD(CA(+)) or HFD(CA(-)). IκBNS-/-/LDLr-/- mice fed HFD(CA(+)) (IκBNS-/-/LDLr-/-(CA(+))) showed a 3.5-fold increase of atherosclerotic lesion size in the aorta compared with LDLr-/-(CA(+)) mice (pâ¯<â¯0.01), whereas there was no difference between LDLr-/-(CA(-)) and IκBNS-/-/LDLr-/-(CA(-)) mice. Immunohistochemical analysis of the aortic root revealed that HFD(CA(+)) significantly increased Mac-3 (macrophage)-positive area by 1.5-fold (pâ¯<â¯0.01) and TLR4, interleukin-6 (IL-6) expression by 1.7-fold (pâ¯<â¯0.05) and 1.5-fold (pâ¯<â¯0.05), respectively, in IκBNS-/-/LDLr-/-(CA(+)) compared with LDLr-/---(CA(+)) mice. Furthermore, active STAT3 (pSTAT3)-positive cells were significantly increased by 1.7-fold in the lesions of IκBNS-/-/LDLr-/-(CA(+)) compared with LDLr-/-(CA(+)) mice (pâ¯<â¯0.01). These findings suggest that IκBNS deficiency and HFD(CA(+)) promote atherogenesis in LDLr-/- mice via TLR4/IL-6/STAT3 pathway. Finally, we showed that the monocytes from peripheral blood of IκBNS-/-/LDLr-/-(CA(+)) mice were found to contain the highest proportion of Ly6Chi monocytes among the four groups, suggesting that lack of IκBNS enhanced inflammation in response to HFD(CA(+)) feeding.CONCLUSIONS:
The present study is the first to demonstrate that the activation of innate immune system using HFD(CA(+)) induced significant inflammation and atherogenesis in IκBNS-/-/LDLr-/- compared with LDLr-/- mice.
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Bases de dados:
MEDLINE
Idioma:
En
Revista:
Int J Cardiol Heart Vasc
Ano de publicação:
2019
Tipo de documento:
Article
País de afiliação:
Japão