A reliable LC-MS/MS method for the quantification of five bioactive saponins of crude and processed Bupleurum scorzonerifolium in rat plasma and its application to a pharmacokinetic study.
Biomed Chromatogr
; 33(9): e4570, 2019 Sep.
Article
em En
| MEDLINE
| ID: mdl-31049981
ABSTRACT
A simple and reliable liquid chromatography-mass spectrometry (LC-MS) method was developed for simultaneous determination of saikosaponin A, saikosaponin B1 , saikosaponin C, saikosaponin D and saikosaponin F in rat plasma using glycyrrhetinic acid as an internal standard (IS). The separation was operated on a Waters BEH C18 column. The mobile phases of gradient elution consisted of acetonitrile (A) and 0.1% aqueous acetic acid (B). The mass spectrometric detection was accomplished in multiple reaction monitoring mode. The five saponins displayed good linearity (r2 > 0.9996). The lower limits of quantitation of saikosaponin A, saikosaponin B1 , saikosaponin C, saikosaponin D and saikosaponin F were determined to be 2.9, 2.3, 3.5, 2.9 and 3.1 ng/mL, respectively. Moreover, the intra- and inter-day precisions of the five saponins showed an RSD within 2.96%, whereas the accuracy (RE) ranged from -2.28 to 2.78%. Finally, the developed method was fully validated and applied to a comparative pharmacokinetic study of the five bioactive saponins in rats following oral administration of crude and vinegar-processed Bupleurum scorzonerifolium.
Palavras-chave
Texto completo:
1
Bases de dados:
MEDLINE
Assunto principal:
Saponinas
/
Medicamentos de Ervas Chinesas
/
Cromatografia Líquida
/
Bupleurum
/
Espectrometria de Massas em Tandem
Tipo de estudo:
Prognostic_studies
Limite:
Animals
Idioma:
En
Revista:
Biomed Chromatogr
Ano de publicação:
2019
Tipo de documento:
Article
País de afiliação:
China