Characterization of recombinant endo-1,4-ß-xylanase of Bacillus halodurans C-125 and rational identification of hot spot amino acid residues responsible for enhancing thermostability by an in-silico approach.

Mahmood, Malik Siddique; Rasul, Faiz; Saleem, Mahjabeen; Afroz, Amber; Malik, Muhammad Faheem; Ashraf, Naeem Mehmood; Rashid, Umar; Naz, Shumaila; Zeeshan, Nadia

Mahmood, Malik Siddique; Rasul, Faiz; Saleem, Mahjabeen; Afroz, Amber; Malik, Muhammad Faheem; Ashraf, Naeem Mehmood; Rashid, Umar; Naz, Shumaila; Zeeshan, Nadia.

Afiliação

Mahmood MS; Institute of Biochemistry and Biotechnology, University of the Punjab, P. O Box No, 54590, Lahore, Pakistan.
Rasul F; Department of Biochemistry and Molecular Biology, University of Science and Technology, Hefei, China.
Saleem M; Institute of Biochemistry and Biotechnology, University of the Punjab, P. O Box No, 54590, Lahore, Pakistan.
Afroz A; Department of Biochemistry and Biotechnology, University of Gujrat, P. O Box No. 50700, Gujrat, Pakistan.
Malik MF; Department of Biochemistry and Biotechnology, University of Gujrat, P. O Box No. 50700, Gujrat, Pakistan.
Ashraf NM; Department of Biochemistry and Biotechnology, University of Gujrat, P. O Box No. 50700, Gujrat, Pakistan.
Rashid U; Department of Biochemistry and Biotechnology, University of Gujrat, P. O Box No. 50700, Gujrat, Pakistan.
Naz S; Department of Biosciences, University of Gujrat, Gujrat, Pakistan.
Zeeshan N; Department of Biochemistry and Biotechnology, University of Gujrat, P. O Box No. 50700, Gujrat, Pakistan. nadia.zeeshan@uog.edu.pk.

Mol Biol Rep ; 46(4): 3651-3662, 2019 Aug.

Article em En | MEDLINE | ID: mdl-31079316

Assuntos

Bacillus/enzimologia; Endo-1,4-beta-Xilanases/genética; Aminoácidos; Bacillus/genética; Sequência de Bases/genética; Clonagem Molecular; Simulação por Computador; Endo-1,4-beta-Xilanases/química; Endo-1,4-beta-Xilanases/isolamento & purificação; Endo-1,4-beta-Xilanases/metabolismo; Estabilidade Enzimática/genética; Cinética; Modelos Moleculares; Proteínas Mutantes/genética; Proteínas Mutantes/metabolismo; Engenharia de Proteínas; Proteínas Recombinantes/química; Proteínas Recombinantes/genética; Proteínas Recombinantes/metabolismo; Temperatura

Palavras-chave

Bacillus halodurans C-125; Cloning and expression; Computational rational design; Endo 1,4-ß-xylanase; Thermostability; Xylanase

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Texto completo: 1 Bases de dados: MEDLINE Assunto principal: Bacillus / Endo-1,4-beta-Xilanases Tipo de estudo: Diagnostic_studies / Prognostic_studies Idioma: En Revista: Mol Biol Rep Ano de publicação: 2019 Tipo de documento: Article País de afiliação: Paquistão

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