A 3D culture model of innervated human skeletal muscle enables studies of the adult neuromuscular junction.
Elife
; 82019 05 14.
Article
em En
| MEDLINE
| ID: mdl-31084710
ABSTRACT
Two-dimensional (2D) human skeletal muscle fiber cultures are ill-equipped to support the contractile properties of maturing muscle fibers. This limits their application to the study of adult human neuromuscular junction (NMJ) development, a process requiring maturation of muscle fibers in the presence of motor neuron endplates. Here we describe a three-dimensional (3D) co-culture method whereby human muscle progenitors mixed with human pluripotent stem cell-derived motor neurons self-organize to form functional NMJ connections. Functional connectivity between motor neuron endplates and muscle fibers is confirmed with calcium imaging and electrophysiological recordings. Notably, we only observed epsilon acetylcholine receptor subunit protein upregulation and activity in 3D co-cultures. Further, 3D co-culture treatments with myasthenia gravis patient sera shows the ease of studying human disease with the system. Hence, this work offers a simple method to model and evaluate adult human NMJ de novo development or disease in culture.
Palavras-chave
Texto completo:
1
Bases de dados:
MEDLINE
Assunto principal:
Técnicas de Cultura de Órgãos
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Músculo Esquelético
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Técnicas de Cocultura
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Junção Neuromuscular
Limite:
Humans
Idioma:
En
Revista:
Elife
Ano de publicação:
2019
Tipo de documento:
Article
País de afiliação:
Canadá