Targeted induction of bone marrow mesenchymal stem cells to have effectiveness on diabetic pancreatic restoration.
In Vitro Cell Dev Biol Anim
; 55(6): 453-461, 2019 Jun.
Article
em En
| MEDLINE
| ID: mdl-31140102
ABSTRACT
Although bone marrow-derived mesenchymal stem cells (BMSCs) have been reported to be effective for the attenuation of diabetes, they have limitations. Whether BMSCs can be target-induced by pancreatic stem cells (PSCs) to have effectiveness for the restoration of diabetic islet injury was unknown. In this study, based on their successful isolation and cultivation, BMSCs were co-cultured with PSCs. The pancreatic stem cells markers, Nestin and Neurogenin3 in co-cultured BMSCs were detected to evaluate the target-induction effects. After the diabetic rats were intravenously injected with the target-induced BMSCs, general indicators and islet morphology were detected. The islet insulin generation, and serum insulin and C-peptide contents were measured. It was found that after co-culture, the mRNA expressions, protein contents and distributions of Nestin and Neurogenin3, were dramatically high in BMSCs, indicating that they were successfully target-induced to pancreatic stem-like cells. Furthermore, the target-induced BMSCs had beneficial effects on serum glycated albumin levels and glycogen contents as well as islet morphology of the diabetic rats. Besides elevation of islet insulin generation, the target-induced BMSCs had significant effect on serum insulin and C-peptide contents. In conclusion, BMSCs could be target-induced by PSCs to have effectiveness on the pancreatic restoration of diabetic rats.
Palavras-chave
Texto completo:
1
Bases de dados:
MEDLINE
Assunto principal:
Pâncreas
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Ilhotas Pancreáticas
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Transplante de Células-Tronco Mesenquimais
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Diabetes Mellitus Experimental
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Células-Tronco Mesenquimais
Limite:
Animals
Idioma:
En
Revista:
In Vitro Cell Dev Biol Anim
Assunto da revista:
BIOLOGIA
Ano de publicação:
2019
Tipo de documento:
Article