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let-7i inhibits proliferation and migration of bladder cancer cells by targeting HMGA1.
Qin, M-M; Chai, X; Huang, H-B; Feng, G; Li, X-N; Zhang, J; Zheng, R; Liu, X-C; Pu, C.
Afiliação
  • Qin MM; Clinical Laboratory, The First Affiliated Hospital of Wannan Medical College, No.2, West Zheshan Road, Wuhu, 241001, Anhui, China.
  • Chai X; Department of Urology, The First Affiliated Hospital of Wannan Medical College, Wuhu, 241001, Anhui, China.
  • Huang HB; Department of Urology, The First Affiliated Hospital of Wannan Medical College, Wuhu, 241001, Anhui, China.
  • Feng G; Clinical Laboratory, The First Affiliated Hospital of Wannan Medical College, No.2, West Zheshan Road, Wuhu, 241001, Anhui, China.
  • Li XN; Clinical Laboratory, The First Affiliated Hospital of Wannan Medical College, No.2, West Zheshan Road, Wuhu, 241001, Anhui, China.
  • Zhang J; Clinical Laboratory, The First Affiliated Hospital of Wannan Medical College, No.2, West Zheshan Road, Wuhu, 241001, Anhui, China.
  • Zheng R; Clinical Laboratory, The First Affiliated Hospital of Wannan Medical College, No.2, West Zheshan Road, Wuhu, 241001, Anhui, China.
  • Liu XC; Clinical Laboratory, The First Affiliated Hospital of Wannan Medical College, No.2, West Zheshan Road, Wuhu, 241001, Anhui, China.
  • Pu C; Clinical Laboratory, The First Affiliated Hospital of Wannan Medical College, No.2, West Zheshan Road, Wuhu, 241001, Anhui, China. philipcpu@163.com.
BMC Urol ; 19(1): 53, 2019 Jun 13.
Article em En | MEDLINE | ID: mdl-31196036
ABSTRACT

BACKGROUND:

Let-7 is one of the earliest discovered microRNAs(miRNAs) and has been reported to be down-regulated in multiple malignant tumors. The effects and molecular mechanisms of let-7i in bladder cancer are still unclear. This study was to investigate the effects and potential mechanisms of let-7i on bladder cancer cells.

METHODS:

Total RNA was extracted from bladder cancer cell lines. The expression levels of let-7i and HMGA1 were examined by quantitative real-time PCR. Cell viability was detected using the CCK-8 and colony formation assays, while transwell and wound healing assays were used to evaluate migration ability. Luciferase reporter assay and western blot were used to confirm the target gene of let-7i.

RESULTS:

Compared with the SV-40 immortalized human uroepithelial cell line (SV-HUC-1), bladder cancer cell lines T24 and 5637 had low levels of let-7i expression, but high levels of high mobility group protein A1 (HMGA1) expression. Transfection of cell lines T24 and 5637 with let-7i mimic suppressed cell proliferation and migration. Luciferase reporter assay confirmed HMGA1 may be one of the target genes of let-7i-5p. Protein and mRNA expression of HMGA1 was significantly downregulated in let-7i mimic transfected cell lines T24 and 5637.

CONCLUSIONS:

Up-regulation of let-7i suppressed proliferation and migration of the human bladder cancer cell lines T24 and 5637 by targeting HMGA1. These findings suggest that let-7i might be considered as a novel therapeutic target for bladder cancer.
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Texto completo: 1 Bases de dados: MEDLINE Assunto principal: Neoplasias da Bexiga Urinária / Movimento Celular / Proteína HMGA1a / MicroRNAs / Proliferação de Células Limite: Humans Idioma: En Revista: BMC Urol Assunto da revista: UROLOGIA Ano de publicação: 2019 Tipo de documento: Article País de afiliação: China

Texto completo: 1 Bases de dados: MEDLINE Assunto principal: Neoplasias da Bexiga Urinária / Movimento Celular / Proteína HMGA1a / MicroRNAs / Proliferação de Células Limite: Humans Idioma: En Revista: BMC Urol Assunto da revista: UROLOGIA Ano de publicação: 2019 Tipo de documento: Article País de afiliação: China