Cryo-EM structure of Neurospora crassa respiratory complex IV.
IUCrJ
; 6(Pt 4): 773-780, 2019 Jul 01.
Article
em En
| MEDLINE
| ID: mdl-31316820
ABSTRACT
In fungi, the mitochondrial respiratory chain complexes (complexes I-IV) are responsible for oxidative phosphorylation, as in higher eukaryotes. Cryo-EM was used to identify a 200â
kDa membrane protein from Neurospora crassa in lipid nanodiscs as cytochrome c oxidase (complex IV) and its structure was determined at 5.5â
Å resolution. The map closely resembles the cryo-EM structure of complex IV from Saccharomyces cerevisiae. Its ten subunits are conserved in S. cerevisiae and Bos taurus, but other transmembrane subunits are missing. The different structure of the Cox5a subunit is typical for fungal complex IV and may affect the interaction with complex III in a respiratory supercomplex. Additional density was found between the matrix domains of the Cox4 and Cox5a subunits that appears to be specific to N. crassa.
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Bases de dados:
MEDLINE
Idioma:
En
Revista:
IUCrJ
Ano de publicação:
2019
Tipo de documento:
Article
País de afiliação:
Alemanha