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Arabidopsis SWR1-associated protein methyl-CpG-binding domain 9 is required for histone H2A.Z deposition.
Potok, Magdalena E; Wang, Yafei; Xu, Linhao; Zhong, Zhenhui; Liu, Wanlu; Feng, Suhua; Naranbaatar, Bilguudei; Rayatpisheh, Shima; Wang, Zonghua; Wohlschlegel, James A; Ausin, Israel; Jacobsen, Steven E.
Afiliação
  • Potok ME; Department of Molecular, Cell and Developmental Biology, University of California, Los Angeles, Los Angeles, CA, 90095, USA.
  • Wang Y; Basic Forestry and Proteomics Center, Fujian Agriculture and Forestry University, Fuzhou, 350002, China.
  • Xu L; Basic Forestry and Proteomics Center, Fujian Agriculture and Forestry University, Fuzhou, 350002, China.
  • Zhong Z; Department of Molecular, Cell and Developmental Biology, University of California, Los Angeles, Los Angeles, CA, 90095, USA.
  • Liu W; Basic Forestry and Proteomics Center, Fujian Agriculture and Forestry University, Fuzhou, 350002, China.
  • Feng S; State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, Fujian Agriculture and Forestry University, Fuzhou, 350002, China.
  • Naranbaatar B; Department of Molecular, Cell and Developmental Biology, University of California, Los Angeles, Los Angeles, CA, 90095, USA.
  • Rayatpisheh S; Zhejiang University-University of Edinburgh Institute, Zhejiang University School of Medicine, Hangzhou, 310058, China.
  • Wang Z; Department of Molecular, Cell and Developmental Biology, University of California, Los Angeles, Los Angeles, CA, 90095, USA.
  • Wohlschlegel JA; Department of Molecular, Cell and Developmental Biology, University of California, Los Angeles, Los Angeles, CA, 90095, USA.
  • Ausin I; Department of Biological Chemistry, David Geffen School of Medicine, University of California, Los Angeles, Los Angeles, CA, 90095, USA.
  • Jacobsen SE; Genomic Institute of the Novartis Research Foundation, 10675 John Jay Hopkins Dr., San Diego, CA, 92121, USA.
Nat Commun ; 10(1): 3352, 2019 07 26.
Article em En | MEDLINE | ID: mdl-31350403
Deposition of the histone variant H2A.Z by the SWI2/SNF2-Related 1 chromatin remodeling complex (SWR1-C) is important for gene regulation in eukaryotes, but the composition of the Arabidopsis SWR1-C has not been thoroughly characterized. Here, we aim to identify interacting partners of a conserved Arabidopsis SWR1 subunit ACTIN-RELATED PROTEIN 6 (ARP6). We isolate nine predicted components and identify additional interactors implicated in histone acetylation and chromatin biology. One of the interacting partners, methyl-CpG-binding domain 9 (MBD9), also strongly interacts with the Imitation SWItch (ISWI) chromatin remodeling complex. MBD9 is required for deposition of H2A.Z at a distinct subset of ARP6-dependent loci. MBD9 is preferentially bound to nucleosome-depleted regions at the 5' ends of genes containing high levels of activating histone marks. These data suggest that MBD9 is a SWR1-C interacting protein required for H2A.Z deposition at a subset of actively transcribing genes.
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Texto completo: 1 Bases de dados: MEDLINE Assunto principal: Histonas / Arabidopsis / Proteínas de Arabidopsis / Histona Acetiltransferases / Proteínas dos Microfilamentos Tipo de estudo: Prognostic_studies / Risk_factors_studies Idioma: En Revista: Nat Commun Assunto da revista: BIOLOGIA / CIENCIA Ano de publicação: 2019 Tipo de documento: Article País de afiliação: Estados Unidos
Texto completo
Adicionar na Minha BVS
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XML
PubMed Links
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Texto completo: 1 Bases de dados: MEDLINE Assunto principal: Histonas / Arabidopsis / Proteínas de Arabidopsis / Histona Acetiltransferases / Proteínas dos Microfilamentos Tipo de estudo: Prognostic_studies / Risk_factors_studies Idioma: En Revista: Nat Commun Assunto da revista: BIOLOGIA / CIENCIA Ano de publicação: 2019 Tipo de documento: Article País de afiliação: Estados Unidos