Optimization and Characterization of Calcium Phosphate Transfection in Mesenchymal Stem Cells.
Tissue Eng Part C Methods
; 25(9): 543-552, 2019 09.
Article
em En
| MEDLINE
| ID: mdl-31441373
ABSTRACT
IMPACT STATEMENT Mesenchymal stem cells (MSCs) are a promising tool for cell therapy, and gene-modified MSCs further expand their applications. To take full advantage of MSCs as a therapeutic approach, developing effective gene transfer methods is critical. Calcium phosphate transfection is well-established and safe, but the protocols need to be optimized according to different cell types. Currently, there is no optimized protocol for MSCs. This study optimized the protocol of calcium phosphate transfection for MSCs and highlighted the importance of serum during the process of transfection. More interestingly, the behavior of gene overexpression in MSCs in the in vivo environment was verified.
Palavras-chave
Texto completo:
1
Bases de dados:
MEDLINE
Assunto principal:
Fosfatos de Cálcio
/
Transfecção
/
Células-Tronco Mesenquimais
Tipo de estudo:
Guideline
Limite:
Animals
/
Humans
/
Male
Idioma:
En
Revista:
Tissue Eng Part C Methods
Assunto da revista:
BIOTECNOLOGIA
/
HISTOLOGIA
Ano de publicação:
2019
Tipo de documento:
Article