Molecular Cloning, Lentiviral Transduction, and Expression of Recombinant ADAMTSL2 and ADAMTSL4.

Singh, Mukti; Cain, Stuart A; Baldock, Clair

Singh, Mukti; Cain, Stuart A; Baldock, Clair.

Afiliação

Singh M; Faculty of Biology, Medicine and Health, Wellcome Centre for Cell-Matrix Research, Division of Cell-Matrix Biology and Regenerative Medicine, School of Biological Sciences, University of Manchester, Manchester Academic Health Science Centre, Manchester, UK.
Cain SA; Faculty of Biology, Medicine and Health, Wellcome Centre for Cell-Matrix Research, Division of Cell-Matrix Biology and Regenerative Medicine, School of Biological Sciences, University of Manchester, Manchester Academic Health Science Centre, Manchester, UK.
Baldock C; Faculty of Biology, Medicine and Health, Wellcome Centre for Cell-Matrix Research, Division of Cell-Matrix Biology and Regenerative Medicine, School of Biological Sciences, University of Manchester, Manchester Academic Health Science Centre, Manchester, UK. clair.baldock@manchester.ac.uk.

Methods Mol Biol ; 2043: 137-155, 2020.

Article em En | MEDLINE | ID: mdl-31463909

Assuntos

Proteínas ADAMTS/genética; Clonagem Molecular/métodos; Transdução Genética/métodos; Proteínas ADAMTS/metabolismo; Vetores Genéticos; Células HEK293; Humanos; Lentivirus/genética; Proteínas Recombinantes/metabolismo

Palavras-chave

HEK293-EBNA; Lentiviral system; Lentiviral transduction; Mammalian; Molecular cloning; Recombinant expression; Stable cell lines

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Texto completo: 1 Bases de dados: MEDLINE Assunto principal: Transdução Genética / Clonagem Molecular / Proteínas ADAMTS Tipo de estudo: Guideline Limite: Humans Idioma: En Revista: Methods Mol Biol Assunto da revista: BIOLOGIA MOLECULAR Ano de publicação: 2020 Tipo de documento: Article País de afiliação: Reino Unido

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