Shiga toxin 2eB-transgenic lettuce vaccine is effective in protecting weaned piglets from edema disease caused by Shiga toxin-producing Escherichia coli infection.
Anim Sci J
; 90(11): 1460-1467, 2019 Nov.
Article
em En
| MEDLINE
| ID: mdl-31502390
ABSTRACT
Porcine edema disease (ED) is a toxemia that is caused by enteric infection with Shiga toxin 2e (Stx2e)-producing Escherichia coli (STEC) and is associated with high mortality. Since ED occurs most frequently during the weaning period, preweaning vaccination of newborn piglets is required. We developed stx2eB-transgenic lettuce as an oral vaccine candidate against ED and examined its protective efficacy using a piglet STEC infection model. Two serially developed Stx2eB-lettuce strains, 2BN containing ingredient Stx2eB constituting a concentration level of 0.53 mg Stx2eB/g of powdered lettuce dry weight (DW) and 2BH containing ingredient Stx2eB constituting a concentration level of 2.3 mg of Stx2eB/g of powdered lettuce DW, were evaluated in three sequential experiments. Taken the results together, oral administration of Stx2eB-lettuce vaccine was suggested to relieve the pathogenic symptoms of ED in piglets challenged with virulent STEC strain. Our data suggested that Stx2eB-lettuce is a promising first oral vaccine candidate against ED.
Palavras-chave
Texto completo:
1
Bases de dados:
MEDLINE
Assunto principal:
Suínos
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Desmame
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Vacinas Bacterianas
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Lactuca
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Toxina Shiga II
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Edematose Suína
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Infecções por Escherichia coli
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Escherichia coli Shiga Toxigênica
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Animais Recém-Nascidos
Limite:
Animals
Idioma:
En
Revista:
Anim Sci J
Assunto da revista:
MEDICINA VETERINARIA
Ano de publicação:
2019
Tipo de documento:
Article
País de afiliação:
Japão