Multimodal Analysis of Cell Types in a Hypothalamic Node Controlling Social Behavior.
Cell
; 179(3): 713-728.e17, 2019 10 17.
Article
em En
| MEDLINE
| ID: mdl-31626771
ABSTRACT
The ventrolateral subdivision of the ventromedial hypothalamus (VMHvl) contains â¼4,000 neurons that project to multiple targets and control innate social behaviors including aggression and mounting. However, the number of cell types in VMHvl and their relationship to connectivity and behavioral function are unknown. We performed single-cell RNA sequencing using two independent platforms-SMART-seq (â¼4,500 neurons) and 10x (â¼78,000 neurons)-and investigated correspondence between transcriptomic identity and axonal projections or behavioral activation, respectively. Canonical correlation analysis (CCA) identified 17 transcriptomic types (T-types), including several sexually dimorphic clusters, the majority of which were validated by seqFISH. Immediate early gene analysis identified T-types exhibiting preferential responses to intruder males versus females but only rare examples of behavior-specific activation. Unexpectedly, many VMHvl T-types comprise a mixed population of neurons with different projection target preferences. Overall our analysis revealed that, surprisingly, few VMHvl T-types exhibit a clear correspondence with behavior-specific activation and connectivity.
Palavras-chave
Texto completo:
1
Bases de dados:
MEDLINE
Assunto principal:
Comportamento Social
/
Hipotálamo
/
Neurônios
Limite:
Animals
Idioma:
En
Revista:
Cell
Ano de publicação:
2019
Tipo de documento:
Article
País de afiliação:
Panamá