Your browser doesn't support javascript.
loading
Ki67 expression and localization of T cells after neoadjuvant therapies as reliable predictive markers in rectal cancer.
Imaizumi, Ken; Suzuki, Toshihiro; Kojima, Motohiro; Shimomura, Manami; Sakuyama, Naoki; Tsukada, Yuichiro; Sasaki, Takeshi; Nishizawa, Yuji; Taketomi, Akinobu; Ito, Masaaki; Nakatsura, Tetsuya.
Afiliação
  • Imaizumi K; Deparment of Colorectal Surgery, National Cancer Center Hospital East, Kashiwa, Japan.
  • Suzuki T; Division of Cancer Immunotherapy, Exploratory Oncology Research & Clinical Trial Center, National Cancer Center, Kashiwa, Japan.
  • Kojima M; Department of Gastroenterological Surgery I, Graduate School of Medicine, Hokkaido University, Sapporo, Japan.
  • Shimomura M; Division of Cancer Immunotherapy, Exploratory Oncology Research & Clinical Trial Center, National Cancer Center, Kashiwa, Japan.
  • Sakuyama N; Division of Pathology, National Cancer Center Hospital East, Kashiwa, Japan.
  • Tsukada Y; Division of Cancer Immunotherapy, Exploratory Oncology Research & Clinical Trial Center, National Cancer Center, Kashiwa, Japan.
  • Sasaki T; Deparment of Colorectal Surgery, National Cancer Center Hospital East, Kashiwa, Japan.
  • Nishizawa Y; Deparment of Colorectal Surgery, National Cancer Center Hospital East, Kashiwa, Japan.
  • Taketomi A; Deparment of Colorectal Surgery, National Cancer Center Hospital East, Kashiwa, Japan.
  • Ito M; Deparment of Colorectal Surgery, National Cancer Center Hospital East, Kashiwa, Japan.
  • Nakatsura T; Department of Gastroenterological Surgery I, Graduate School of Medicine, Hokkaido University, Sapporo, Japan.
Cancer Sci ; 111(1): 23-35, 2020 Jan.
Article em En | MEDLINE | ID: mdl-31660687
ABSTRACT
Chemoradiotherapy (CRT) is the standard neoadjuvant therapy for locally advanced rectal cancer (RC). However, neoadjuvant chemotherapy (NAC) also shows favorable outcomes. Although the immunological environment of RC has been thoroughly discussed, the effect of NAC on it is less clear. Here, we investigated the immunological microenvironment, including T cell infiltration, activation, and topological distribution, of resected RC tissue after neoadjuvant therapies and evaluated the correlation between T cell subsets and patient prognosis. Rectal cancer patients (n = 188) were enrolled and categorized into 3 groups, namely CRT (n = 41), NAC (n = 46), and control (surgery alone; n = 101) groups. Characterization of residual carcinoma cells and T cell subsets in resected tissues was performed using multiplex fluorescence immunohistochemistry. The densities of total and activated (Ki67high ) T cells in tissues after NAC, but not CRT, were higher than in control. In both CRT and NAC groups, patients presenting with higher treatment effects showed aggressive infiltration of T cell subsets into carcinomas. Multivariate analyses of pathological and immunological features and prognosis revealed that carcinoma Ki67high CD4+ T cells after CRT and stromal Ki67high CD8+ T cells after NAC are important prognostic factors, respectively. Our results suggest that evaluation of T cell activation with Ki67 expression and its tumor localization can be used to determine the prognosis of advanced RC after neoadjuvant therapies.
Assuntos
Palavras-chave

Texto completo: 1 Bases de dados: MEDLINE Assunto principal: Neoplasias Retais / Linfócitos T CD4-Positivos / Biomarcadores Tumorais / Linfócitos T CD8-Positivos / Antígeno Ki-67 Tipo de estudo: Prognostic_studies / Risk_factors_studies Limite: Adult / Aged / Aged80 / Female / Humans / Male / Middle aged Idioma: En Revista: Cancer Sci Ano de publicação: 2020 Tipo de documento: Article País de afiliação: Japão

Texto completo: 1 Bases de dados: MEDLINE Assunto principal: Neoplasias Retais / Linfócitos T CD4-Positivos / Biomarcadores Tumorais / Linfócitos T CD8-Positivos / Antígeno Ki-67 Tipo de estudo: Prognostic_studies / Risk_factors_studies Limite: Adult / Aged / Aged80 / Female / Humans / Male / Middle aged Idioma: En Revista: Cancer Sci Ano de publicação: 2020 Tipo de documento: Article País de afiliação: Japão