Atoms to Phenotypes: Molecular Design Principles of Cellular Energy Metabolism.
Cell
; 179(5): 1098-1111.e23, 2019 11 14.
Article
em En
| MEDLINE
| ID: mdl-31730852
ABSTRACT
We report a 100-million atom-scale model of an entire cell organelle, a photosynthetic chromatophore vesicle from a purple bacterium, that reveals the cascade of energy conversion steps culminating in the generation of ATP from sunlight. Molecular dynamics simulations of this vesicle elucidate how the integral membrane complexes influence local curvature to tune photoexcitation of pigments. Brownian dynamics of small molecules within the chromatophore probe the mechanisms of directional charge transport under various pH and salinity conditions. Reproducing phenotypic properties from atomistic details, a kinetic model evinces that low-light adaptations of the bacterium emerge as a spontaneous outcome of optimizing the balance between the chromatophore's structural integrity and robust energy conversion. Parallels are drawn with the more universal mitochondrial bioenergetic machinery, from whence molecular-scale insights into the mechanism of cellular aging are inferred. Together, our integrative method and spectroscopic experiments pave the way to first-principles modeling of whole living cells.
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Bases de dados:
MEDLINE
Assunto principal:
Células
/
Metabolismo Energético
Idioma:
En
Revista:
Cell
Ano de publicação:
2019
Tipo de documento:
Article